Method of purifying 3-hyroxyalkanoic acid copolymer
View Patent ↗The object of this invention is to provide a method for purifying PHA separated from PHA-containing microbial cells in high purity without incurring any serious decrease in molecular weight. The present invention provides a method for purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism, which comprises treating an aqueous suspension containing the 3-hydroxyalkanoic acid copolymer separated from a microorganism with a hydrogen peroxide while controlling the pH of said aqueous suspension by adding an alkali either continuously or intermittently.
1. A method for purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism,
which comprises treating an aqueous suspension containing the 3-hydroxyalkanoic acid copolymer separated from a microorganism with hydrogen peroxide while controlling the pH of said aqueous suspension by adding an alkali either continuously or intermittently,
wherein the pH of the aqueous suspension is controlled to be between 8 and 13.
2. The purification method according to claim 1 ,
wherein the concentration of hydrogen peroxide in the aqueous suspension is in a range of 0.01 to 1% by weight.
3. The purification method according to claim 1 ,
wherein the 3-hydroxyalkanoic acid copolymer is a copolymer of D-3-hydroxyhexanoate and one or more other D-3-hydroxyalkanoic acids.
4. The purification method according to claim 1 ,
wherein the 3-hydroxyalkanoic acid copolymer is a copolymer constituted of at least two species of monomers selected from a group consisting of 3-hydroxypropionate, 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxyhexanoate, 3-hydroxyheptanoate and 3-hydroxyoctanoate.
5. The purification method according to claim 1 ,
wherein the 3-hydroxyalkanoic acid copolymer is a binary copolymer derived from D-3-hydroxyhexanoate and D-3-hydroxybutyrate, or a ternary copolymer derived from D-3-hydroxyhexanoate, D-3-hydroxybutyrate and D-3-hydroxyvalerate.
6. The purification method according to claim 1 ,
wherein the microorganism producing the 3-hydroxyalkanoic acid copolymer is a microorganism belonging to the genus Aeromonas.
7. The purification method according to claim 6 ,
wherein the microorganism producing the 3-hydroxyalkanoic acid copolymer is Aeromonas caviae or Aeromonas hydrophila.
8. The purification method according to claim 1 ,
wherein the microorganism producing the 3-hydroxyalkanoic acid copolymer is a cell strain of microorganism transformed by a poly-3-hydroxyalkanoic acid synthase group gene derived from Aeromonas caviae.
9. The purification method according to claim 1 ,
wherein the aqueous suspension of the 3-hydroxyalkanoic acid copolymer is obtainable by;
solubilizing the total or part of cell constituent substances
other than the 3-hydroxyalkanoic acid copolymer to separate the 3-hydroxyalkanoic acid copolymer by adding an alkali simultaneously with physical disruption while stirring a suspension of a 3-hydroxyalkanoic acid copolymer-containing strain, and
suspending the 3-hydroxyalkanoic acid copolymer in water.