Bioinformatically detectable group of novel viral regulatory genes and uses thereof
The present invention relates to a first group of novel genes, here identified as genomic address messenger or VGAM genes, and a second group of novel operon-like genes, here identified as viral genomic record or VGR genes. VGAM genes selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 1560 VGAM genes, and 205 VGR genes, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting VGAM and VGR genes and specific functions and utilities thereof, for detecting expression of VGAM and VGR genes, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
1 . An isolated viral DNA encoding:
RNA comprising about 50 to about 120 nucleotides,
wherein about 18 to about 24 nucleotides at the 5′ of the RNA are a partial inversed-reversed sequence of a sequence at the 3′ of the RNA, and wherein
a portion of the RNA is a partial inversed-reversed sequence of a portion of a binding site associated with at least one host target gene.
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3 . The DNA of claim 1 wherein the RNA is capable of modulating expression of the target gene.
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6 . The DNA of claim 1 wherein the untranslated region of an mRNA encoded by the target gene comprises the binding site.
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8 . A vector capable of expressing the DNA of claim 1 .
9 . A method of inhibiting translation of a host target gene comprising introducing the vector of claim 8 into the host.
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12 . A probe comprising a sequence complementary to a portion of the RNA encoded by the DNA of claim 1 .
13 . A method of detecting expression of a viral miRNA, comprising detecting hybridization by a probe of claim 12 , wherein the probe comprises a sequence complementary to a portion of the miRNA.
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18 . A method for treating infection by a virus in a host comprising introducing to a host in need thereof a RNA comprising a sequence characterized by the following:
(a) the sequence is complementary to a portion of the miRNA expressed by the virus;
(b) the sequence is complementary to a portion of a binding site of a miRNA expressed by the virus;
(c) the sequence is complementary to a portion of a host miRNA characterized by increased expression with infection by the virus; or
(d) the sequence is complementary to a portion of a binding site in a host miRNA encoding a protein characterized by increased expression with infection by the virus.
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