Triazine compounds and their use in forming multidimensional libraries for affinity chromatography

1. A compound comprising affinity ligands wherein the compound is immobilized on a support matrix, and wherein the compound, together with the support matrix, is represented by the formula

wherein each Z is the same or different and is

wherein each X is independently selected from —NH— and diaminoalkane, diethylenetriamine or tris(aminoethyl)amine spacers linked to the triazine rings by amine groups,

each Y is independently selected from optionally substituted aliphatic and aromatic primary amines, and the Y groups provide the affinity ligands; and

M is a support matrix.

2. The compound according to claim 1 , of the formula

3. The compound according to claim 2 , wherein either or each Z is Y.

4. The compound according to claim 1 , wherein each X independently represents a secondary amino group or a diaminoalkane.

5. The compound according to claim 1 , of the formula

6. A method for the synthesis of a compound comprising affinity ligands wherein the compound is immobilized on a support matrix, and wherein the compound, together with the support matrix, is represented by the formula

wherein each Z is the same or different and is

wherein each X is independently selected from —NH— and diaminoalkane, diethylenetriamine or tris(aminoethyl)amine spacers linked to the triazine rings by amine groups;

each Y is independently selected from optionally substituted aliphatic and aromatic primary amines, and the Y groups provide the affinity ligands; and

M is a support matrix:

wherein said method comprises the reaction of a compound of the formula

wherein each Z is the same or different and is

with an amine-containing support matrix.

7. A library of compounds of the formula:

wherein each Z is the same or different and is

wherein each X is independently selected from —NH— and diaminoalkane, diethylenetriamine or tris(aminoethyl)amine spacers linked to the triazine rings by amine groups;

each Y is independently selected from optionally substituted aliphatic and aromatic primary amines, and the Y groups provide the affinity ligands; and

M is a support matrix.

8. A method for the production of a library of compounds of the formula:

wherein each Z is the same or different and is

wherein each X is independently selected from —NH— and diaminoalkane, diethylenetriamine or tris(aminoethyl)amine spacers linked to the triazine rings by amine groups; each Y is independently selected from optionally substituted aliphatic and aromatic primary amines, and the Y groups provide the affinity ligands; and

M is a support matrix;

wherein said method comprises the synthesis of intermediate structures, either singly or in multiples, dividing the structures into smaller portions, and carrying out appropriate subsequent reaction steps.

9. A method for the separation, isolation, and/or purification of peptides and proteins from a preparation of biological or pharmaceutical compound wherein said method comprises the use of a compound of the formula

wherein each Z is the same or different and is

wherein each X is independently selected from —NH— and diaminoalkane, diethylenetriamine or tris(aminoethyl)amine spacers linked to the triazine rings by amine groups;

each Y is independently selected from optionally substituted aliphatic and aromatic primary amines, and the Y groups provide the affinity ligands; and

M is a support matrix.

10. The method, according to claim 9 , which comprises subjecting a sample containing a proteinaceous material to affinity chromatography using said compound.

11. The process according to claim 10 , wherein the proteinaceous material is an immunoglobulin or a subclass, fragment, precursor or derivative thereof, including fusion proteins, whether derived from natural or recombinant sources.

12. The method according to claim 9 , for the removal of contaminants, including toxic or pathogenic entities, from a preparation of biological or pharmaceutical compound.

13. The library, according to claim 7 , wherein the compounds are on a common support.

14. The compound according to claim 1 , which contains 2 or more triazine rings and 3 independently available Y groups.

15. The compound according to claim 1 , which contains 3 or more triazine rings and 4 independently available Y groups.