IP Library › Granted Patent US 8,017,745

Granted Patent B2

US 8,017,745 · App. 10/537,642 · Granted Sep 13, 2011

Patent

Assignees: Epimmune Inc.; The United States of America as represented by the Secretary of the Navy

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Quick Facts

Patent No.: US 8,017,745
App. No.: 10/537,642
Granted: Sep 13, 2011
Kind: B2

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Abstract

The subject invention provides novel Plasmodium falciparum antigens and novel polynucleotides encoding these antigens. Also provided by the subject invention are methods of using these antigens and polynucleotides.

Claims (47)

1. An isolated or purified polynucleotide: a) encoding a polypeptide comprising SEQ ID NO: 1; b) encoding a Human Leukocyte Antigen (HLA) binding fragment of SEQ ID NO: 1, wherein said HLA binding fragment comprises the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37); or c) that is complementary along the full length of said polynucleotide of a) or b).

2. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said polypeptide comprising SEQ ID NO: 1.

3. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment.

4. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide is complementary along the full length of said polynucleotide of a).

5. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide is complementary along the full length of said polynucleotide of b).

6. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment has a length selected from the group consisting of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, and 35 amino acids.

7. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment consists of the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37).

8. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28).

9. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29).

10. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30).

11. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32).

12. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33).

13. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34).

14. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35).

15. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36).

16. The isolated or purified polynucleotide according to claim 1 , wherein said polynucleotide encodes said HLA binding fragment or is complementary along the full length of said polynucleotide of b), and wherein said HLA binding fragment comprises Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37).

17. A vector comprising a promoter operably linked to a polynucleotide:

a) encoding a polypeptide comprising SEQ ID NO: 1; b)

encoding a Human Leukocyte Antigen (HLA) binding fragment of SEQ ID NO: 1, wherein said HLA binding fragment comprises the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37); or

c) that is complementary along the full length of said polynucleotide of a) or b).

18. The vector according to claim 17 , wherein said polynucleotide encodes said polypeptide comprising SEQ ID NO: 1.

19. The vector according to claim 17 , wherein said polynucleotide encodes said HLA binding fragment.

20. The vector according to claim 17 , wherein said polynucleotide is complementary along the full length of said polynucleotide of a).

21. The vector according to claim 17 , wherein said polynucleotide is complementary along the full length of said polynucleotide of b).

22. The vector according to claim 17 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment has a length selected from the group consisting of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, and 35 amino acids.

23. The vector according to claim 17 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment consists of the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37).

24. An isolated transformed host cell comprising a polynucleotide:

a) encoding a polypeptide comprising SEQ ID NO: 1;

b) encoding a Human Leukocyte Antigen (HLA) binding fragment of SEQ ID NO: 1, wherein said HLA binding fragment comprises the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37); or

c) that is complementary along the full length of said polynucleotide of a) or b).

25. The isolated transformed host cell according to claim 24 , wherein said polynucleotide encodes said polypeptide comprising SEQ ID NO: 1.

26. The isolated transformed host cell according to claim 24 , wherein said polynucleotide encodes said HLA binding fragment.

27. The isolated transformed host cell according to claim 24 , wherein said polynucleotide is complementary along the full length of said polynucleotide of a).

28. The isolated transformed host cell according to claim 24 , wherein said polynucleotide is complementary along the full length of the polynucleotide of b).

29. An isolated transformed host cell according to claim 24 , wherein said polynucleotide is a vector comprising a promoter operably linked to a polynucleotide:

a) encoding a polypeptide comprising SEQ ID NO: 1;

c) that is complementary along the full length of said polynucleotide of a) or b).

30. The isolated transformed host cell according to claim 29 , wherein said polynucleotide encodes said polypeptide comprising SEQ ID NO: 1.

31. The isolated transformed host cell according to claim 29 , wherein said polynucleotide encodes said HLA binding fragment.

32. The isolated transformed host cell according to claim 29 , wherein said polynucleotide is complementary along the full length of said polynucleotide of a).

33. The isolated transformed host cell according to claim 29 , wherein said polynucleotide is complementary along the full length of said polynucleotide of b).

34. The isolated transformed host cell according to claim 29 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment has a length selected from the group consisting of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, and 35 amino acids.

35. The isolated transformed host cell according to claim 29 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment consists of the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37).

36. A method of making a polypeptide comprising culturing an isolated transformed host cell according to claim 24 under conditions that allow for the production of said polypeptide.

37. The isolated transformed host cell according to claim 24 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment has a length selected from the group consisting of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, and 35 amino acids.

38. The isolated transformed host cell according to claim 24 , wherein said polynucleotide encodes said HLA binding fragment, and wherein said HLA binding fragment consists of the amino acid sequence selected from Lys-Thr-Asn-Lys-Trp-Glu-Asp-Ile-Tyr (SEQ ID NO:28), Lys-Ser-Ile-Tyr-Ile-Phe-Tyr-Thr-Tyr (SEQ ID NO:29), Gly-Thr-Phe-Thr-Phe-Gln-Asn-Met-Tyr (SEQ ID NO:30), Tyr-Phe-Glu-Cys-Ile-Met-Lys-Leu-Tyr (SEQ ID NO:32), Val-Tyr-Glu-Gly-Lys-Leu-Lys-Lys-Tyr (SEQ ID NO:33), Val-Val-Asp-Leu-Phe-Cys-Gly-Val-Gly-Tyr (SEQ ID NO:34), Phe-Ser-Ser-Ile-Asn-Thr-Tyr-Asp-Tyr (SEQ ID NO:35), Val-Ser-Asn-Val-Glu-Asp-Ser-Asn-Tyr (SEQ ID NO:36), or Asn-Ser-Asn-Tyr-Asn-Lys-Lys-Leu-Tyr (SEQ ID NO:37).

Assignments (4)

CHANGE OF NAME Recorded Jun 3, 2011

From: PHARMEXA INC.

To: EPIMMUNE INC.

Reel/Frame 026383/0838 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 19, 2006

From: SETTE, ALESSANDRO; SIDNEY, JOHN; SOUTHWOOD, SCOTT; CARUCCI, DANIEL; DOOLAN, DENISE

To: EPIMMUNE INC.; UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY

Reel/Frame 017494/0365 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 6, 2006

From: IDM PHARMA, INC.

To: PHARMEXA INC.