Methods of predicting mortality risk by determining telomere length
The present invention provides for methods of determining telomere length of an organism and correlating the measured telomere length with mortality risk associated with telomere length in a population. The presence of shorter telomeres is associated with an increased mortality rate and increased susceptibility to certain types of diseases for an individual member of a human population.
1. A method for determining mortality risk of a human, said method comprising:
a) determining telomere length in total genomic DNA prepared from a somatic cell of said human; and
b) comparing said telomere length with somatic cell telomere length in an age-matched population of the human, wherein a human having a shorter telomere length compared to the age-matched control is a human with an increased mortality risk,-wherein said mortality risk is associated with vascular disease;
wherein the average telomere length is determined by polymerase chain reaction using a first primer pair to amplify telomere repeat sequences and a second primer pair to amplify a target nucleic acid that is a single copy gene; and
wherein the first primer pair consists of tel 1b (SEQ ID NO: 6) and tel 2b (SEQ ID NO: 7).
2. The method according to claim 1 , wherein telomere length is the average telomere length.
3. The method according to claim 1 , wherein said telomere length is determined from blood.
4. The method according to claim 1 , wherein said telomere length is determined from lymphoid cells.
5. The method according to claim 4 , wherein said lymphoid cells comprise T cells.
6. The method according to claim 1 , wherein
said aged matched population is within about 10 human years of the age of said organism.
7. The method according to claim 6 , wherein said aged matched population is within about 5 years of the age of said human.
8. The method of claim 1 , wherein the single copy gene is the 36B4 gene encoding acidic ribosomal phosphoprotein PO.
9. The method of claim 8 , wherein second primer pair to amplify a single copy gene consists of 36B4u (SEQ ID NO: 8) and 36B4d (SEQ ID NO: 9.
10. A method for determining mortality risk of a human, said method comprising:
a) determining the rate of telomere length decrease in total genomic DNA prepared from in a somatic cell of said human; and
b) comparing said rate of decrease with rate of telomere length decrease in the somatic cells of an age-matched population of the human, wherein a human having a higher rate of telomere length decrease compared to the age-matched control is a human with an increased mortality risk, wherein said mortality risk is associated with vascular disease;
wherein the rate of teleomere length decrease is determined by polymerase chain reaction using a first primer pair to amplify telomere repeat sequences and a second primer pair to amplify a single copy gene; and
wherein the first primer pair consists of tel 1b (SEQ ID NO: 6) and tel 2b (SEQ ID NO: 7).