IP Library › Granted Patent US 7,319,014
Granted Patent B2
US 7,319,014 · App. 10/545,905 · Granted Jan 15, 2008

Overexpression, purification and characterization of a thermolabile phosphatase

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Quick Facts
Patent No.
US 7,319,014
App. No.
10/545,905
Granted
Jan 15, 2008
Kind
B2
Abstract

Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.

Claims (3)

1. A method of dephosphorylating a phosphorylated substrate, comprising:

(a) adding the thermolabile Antarctic phosphatase (TAP) of SEQ ID NO: 10 to the substrate in a buffer at pH 5.5-6.5; and

(b) incubating the mixture comprising the substrate and the TAP under conditions to allow phosphate to be removed from the substrate.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 17, 2005
From: GUTHRIE, ELLEN; DAVIS, THEODORE; BENNER, JACK II
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 017831/0400 →