Aminion-origin medical material and method of preparing the same
It is intended to provide an amnion-origin medical material which can be easily handled and fully sterilized and, moreover, favorably acts as a base material for forming a cell layer thereon. This material is prepared by: (i) removing the epithelial layer from the amnion while remaining at at leaset a part of the base membrane thereof; and (ii) drying it under such conditions that the remaining base membrane can sustain a structure allowing the adhesion and proliferation of cells thereon in using.
1 . A medical material derived from amniotic membrane, comprising the following properties (1) to (3) of:
(1) being in a dried state;
(2) not having an epithelial layer; and
(3) having a basement membrane that retains a structure allowing the adhesion and proliferation of cells thereon in use.
2 . The medical material derived from amniotic membrane according to claim 1 , further comprising the following property (4) that:
(4) the medical material is contained in a container in a state that is not substantially in contact with oxygen.
3 . The medical material derived from amniotic membrane according to claim 1 , wherein the state that is not substantially in contact with oxygen is a state in which the container has been evacuated or a state in which the gas in container has been replaced by nitrogen gas.
4 . The medical material derived from amniotic membrane according to claim 1 , wherein the cell is a corneal epithelial cell, a corneal endothelial cell, or an oral mucosal epithelial cell.
5 . A method of preparing a medical material derived from amniotic membrane, the method comprising the steps (i) and (ii):
(i) removing an epithelial layer from amniotic membrane with at least a part of a basement membrane left; and
(ii) drying the amniotic membrane that does not contain the epithelium obtained in the step (i) under such conditions that the remaining basement membrane retains a structure allowing the adhesion and proliferation of cells thereon in use.
6 . The method of preparing a medical material derived from amniotic membrane according to claim 5 , wherein the step (ii) is carried out by lyophilization.
7 . The method of preparing a medical material derived from amniotic membrane according to claim 5 , further comprising the following step (iii):
(iii) containing the dried material obtained in the step (ii) in a container in a state that is not substantially in contact with oxygen.
8 . The method of preparing a medical material derived from amniotic membrane according to claim 7 , wherein the state that is not substantially in contact with oxygen is a state in which the container has been evacuated or a state in which the gas in container has been replaced by nitrogen gas.
9 . A method of constructing a corneal epithelium-like sheet, the method comprising the following step:
cultivating corneal epithelial cells on the medical material according to claim 1 .
10 . A method of constructing a corneal epithelium-like sheet, the method comprising the following steps (a) and (b):
a) cultivating corneal epithelial cells or cells having differentiation potency into corneal epithelial cell-like cells on the medical material according to claim 1; and
b) after cells were proliferated and stratified, bringing an outermost layer of the stratified cells into contact with air.
11 . The method of constructing a corneal epithelium-like sheet according to claim 10 , wherein the step (a) is carried out in the coexistence of supporter cells.
12 . The method of constructing a corneal epithelium-like sheet according to claim 10 , wherein the step (a) is carried out in the coexistence of supporter cells and in a state in which an isolation membrane having a pore size through which the supporter cells cannot pass is provided between the supporter cells and the medical material.
13 . The method of constructing a corneal epithelium-like sheet according to claim 10 , wherein the cell cultivated in the step (a) is an oral mucosal epithelial cell.
14 . A method of constructing a corneal endothelium-like sheet, the method comprising the following steps (A) to (C):
(A) cultivating and proliferating collected corneal endothelial cells;
(B) preparing a cell suspension by collecting the proliferated corneal endothelial cells; and
(C) plating the cell suspension on the medical material according to claim 1 , and cultivating thereof.
15 . The method of constructing a corneal endothelium-like sheet according to claim 14 , wherein the following step (B-1) is carried out after the step (B):
(B-1) increasing a cell density of the cell suspension by centrifugation.
16 . The method of constructing a corneal endothelium-like sheet according to claim 14 , wherein, in the step (C), centrifugation is carried out after the cell suspension is plated.
17 . The method of constructing a corneal endothelium-like sheet according to claim 14 , wherein the step (C) comprise the following steps (C-1) to (C-5):
(C-1) placing a container in a culture container, the container having a bottom surface including a membrane with a pore size allowing a culture solution to pass through;
(C-2) placing the medical material, derived from amniotic membrane and having the properties of: (1) being in a dried state; (2) not having an epithelial layer; and (3) having a basement membrane that retains a structure allowing the adhesion and proliferation of cells thereon in use, on the bottom surface of the container;
(C-3) plating the cell suspension on the medical material;
(C-4) carrying out centrifugation; and
(C-5) cultivating.