Biologic Replacement for Fibrin Clot
The invention provides methods and devices for repairing a ruptured ligament, meniscus, cartilage, tendon, and bone. Methods and products for delivering nucleic acids to damaged tissue are also disclosed.
1 - 70 . (canceled)
71 . A method for in situ gene transfer comprising:
applying a hydrogel containing a non-viral gene transfer vehicle to a tissue site, wherein the tissue is not bone, and
promoting cell migration into the hydrogel to accomplish gene transfer into the cell.
72 . The method of claim 71 , wherein a plurality of cells migrate into the hydrogel and gene transfer continues for one week.
73 - 74 . (canceled)
75 . The method of claim 71 , wherein the gene is expressed by the cell.
76 . The method of claim 75 , wherein gene expression is detectable after one week.
77 - 78 . (canceled)
79 . The method of claim 71 , wherein the hydrogel is a collagen hydrogel.
80 - 84 . (canceled)
85 . The method of claim 71 , wherein the tissue is a intra or extra articular tissue.
86 . The method of claim 85 , wherein the intra or extra articular tissue is a damaged ligament.
87 . (canceled)
88 . The method of claim 86 , wherein the damaged ligament is an anterior cruciate ligament.
89 . The method of claim 85 , wherein the intra or extra articular tissue is a tendon.
90 . The method of claim 85 , wherein the intra or extra articular tissue is a meniscus.
91 . The method of claim 85 , wherein the intra or extra articular tissue is cartilage.
92 - 97 . (canceled)
98 . A method for in situ gene transfer comprising:
applying a hydrogel containing a viral gene transfer vehicle to a tissue site, wherein the tissue is not a tendon, and
promoting cell migration into the hydrogel to accomplish gene transfer in to the cell, wherein the hydrogel forms a scaffold for tissue repair.
99 . The method of claim 98 , wherein the hydrogel is a collagen hydrogel.
100 . (canceled)
101 . The method of claim 98 , wherein the tissue is a intra or extra articular tissue.
102 . The method of claim 101 , wherein the intra or extra articular tissue is a damaged ligament.
103 . (canceled)
104 . The method of claim 102 , wherein the damaged ligament is an anterior cruciate ligament.
105 - 107 . (canceled)
108 . A method for in situ gene transfer comprising:
applying a hydrogel containing a non-nucleic acid based gene transfer vehicle to a tissue site, and
promoting cell migration into the hydrogel to accomplish gene transfer into the cell.
109 . The method of claim 108 , wherein the hydrogel is a collagen hydrogel.
110 . (canceled)
111 . The method of claim 108 , wherein the tissue is a intra or extra articular tissue.
112 . The method of claim 111 , wherein the intra or extra articular tissue is a damaged ligament.
113 - 117 . (canceled)
118 . A composition comprising,
a hydrogel containing a gene transfer vehicle, wherein the hydrogel is free of cells and
wherein the hydrogel includes soluble type I collagen, a plurality of platelets and a neutralizing agent.
119 . The composition of claim 118 , wherein the gene transfer vehicle is a virus.
120 . The composition of claim 118 , wherein the gene transfer vehicle is a plasmid.
121 - 123 . (canceled)