Methods and means for increasing the tolerance of plants to stress conditions
View Patent ↗Methods and means are provided to increase the tolerance of plants to abiotic stress or adverse growing conditions, including drought, high light intensities, high temperatures, nutrient limitations and the like by reducing the activity of endogenous PARG proteins in plants.
1. A method of producing an Arabidopsis, Brassica or tobacco plant tolerant to high light stress conditions, comprising the steps of:
(a) transforming plant cells from an Arabidopsis, Brassica or tobacco plant with a chimeric gene to create transgenic plant cells, said chimeric gene comprising in sequence the following operably linked DNA fragments:
(i) a plant-expressible promoter;
(ii) a DNA region, which when transcribed yields a ParG inhibitory RNA molecule, said ParG inhibitory RNA molecule comprising a sense nucleotide sequence of at least 163 consecutive nucleotides of a coding region comprising the nucleotide sequence of SEQ ID No: 3 from the nucleotide at position 973 to the nucleotide at position 1135 and said ParG inhibitory RNA molecule further comprising an antisense nucleotide sequence of at least 163 consecutive nucleotides of said coding region, wherein said sense and antisense nucleotide sequences are capable of forming a double stranded RNA region comprising said at least 163 consecutive nucleotides; and
(iii) a 3′ end region involved in transcription termination and polyadenylation;
(b) regenerating a population of transgenic plant lines from said transgenic plant cells wherein said chimeric gene is transcribed to yield said ParG inhibitory RNA molecule; and
(c) identifying a plant line within said population of transgenic plant lines, which is tolerant to high light stress conditions as compared to an Arabidopsis, Brassica , or tobacco plant that does not comprise said chimeric gene.