Generation of plants with altered oil content
View Patent ↗The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO103.1 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.
1. A transgenic plant comprising a plant transformation vector comprising a nucleotide sequence that encodes a HIO103.1 polypeptide comprising:
a) the amino acid sequence set forth as SEQ ID NO: 2; or
b) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2;
whereby the transgenic plant has a higher oil content relative to a plant of the same species that does not comprise the plant transformation vector.
2. The transgenic plant of claim 1 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
3. A plant part obtained from the plant according to claim 1 .
4. The plant part of claim 3 , which is a seed.
5. A method of producing oil comprising growing the transgenic plant of claim 1 and recovering oil from said plant.
6. A method of producing a high oil phenotype in a plant, said method comprising: a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a HIO103.1 polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2; or an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2; and b) growing the transformed progenitor cells to produce a transgenic plant,
wherein said nucleotide sequence is expressed, and said transgenic plant exhibits a higher oil content relative to a plant of the same species that does not comprise the plant transformation vector, thereby producing a high oil phenotype.
7. A plant obtained by a method of claim 6 .
8. The plant of claim 7 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
9. The transgenic plant of claim 1 , wherein the nucleotide sequence encodes a HIO103.1 polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
10. The transgenic plant of claim 9 , wherein the nucleotide sequence encodes a HIO103.1 polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
11. The transgenic plant of claim 10 , wherein the nucleotide sequence that encodes a HIO103.1 polypeptide consists of the amino acid sequence set forth as SEQ ID NO: 2.
12. The method of claim 6 , wherein the nucleotide sequence encodes a HIO103.1 polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
13. The method of claim 12 , wherein the nucleotide sequence encodes a HIO103.1 polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
14. The method of claim 13 , wherein the nucleotide sequence that encodes a HIO103.1 polypeptide consists of the amino acid sequence set forth as SEQ ID NO: 2.