Microorganism staining agent and use thereof
View Patent ↗A microorganism staining agent characterized in that an alkali substance and a diazo dye or xanthene dye are contained therein; and a method of detecting microorganisms with the use of the agent. There are provided a microorganism staining agent which resolves the problem of pretreatment posed by the conventional direct speculum method, being capable of staining microorganisms in shorter time; and a method of detecting microorganisms with the use of the agent.
1. A composition for staining a microorganism comprising:
15 to 30 wt. % of an alkali substance; and
Chicago Sky Blue 6B.
2. The composition according to claim 1 , further comprising:
0 to 20 wt. % of methanol; and
0 to 2 wt. % of dimethyl sulfoxide, and
wherein the amount of said Chicago Sky Blue 6B ranges from 0.1 to 1 wt. %.
3. The composition according to claim 2 , wherein the amount of said methanol ranges from 10 to 20 wt. %.
4. The composition according to claim 2 , wherein the amount of said dimethyl sulfoxide ranges from 1 to 2 wt. %.
5. The composition according to claim 1 , wherein said microorganism is fungi.
6. The composition according to claim 5 , wherein said fungi is a member of a genus selected from the group consisting of Trichophyton, Candida and Malassezia.
7. A method for detecting a microorganism in a sample comprising:
contacting said microorganism with the composition of claim 1 to yield a stained microorganism; and
detecting said stained microorganism by direct microscopy.
8. The method according to claim 7 , further comprising:
heating said sample to a temperature ranging from 60° C. to 80° C. for a period ranging from 2 minutes to 5 minutes after said contacting, but before said detecting.
9. The method according to claim 7 , wherein said sample is obtained from a foot, a nail plate, a nail bed, a hair, a dander, a vesicle, and a papula.
10. The method according to claim 7 , wherein said microorganism is fungi.
11. The method according to claim 10 , wherein said fungi is a member of a genus selected from the group consisting of Trichophyton, Candida and Malassezia.
12. A composition for staining a microorganism comprising:
15 to 30 wt. % of an alkali substance; and
Rhodamine B.
13. The composition according to claim 12 , further comprising:
0 to 30 wt. % of methanol; and
5 to 20 wt. % of dimethyl sulfoxide, and
wherein the amount of said Rhodamine B ranges from 0.005 to 0.5 wt. %.
14. The composition according to claim 13 , wherein the amount of said methanol ranges from 10 to 30 wt. %.
15. The composition according to claim 13 , wherein the amount of said dimethyl sulfoxide ranges from 10 to 20 wt. %.
16. The composition according to claim 12 , wherein said microorganism is fungi.
17. The composition according to claim 16 , wherein said fungi is a member of a genus selected from the group consisting of Trichophyton, Candida and Malassezia.
18. A method for detecting a microorganism in a sample comprising:
contacting said microorganism with the composition of claim 12 to yield a stained microorganism; and
detecting said stained microorganism by direct microscopy.
19. The method according to claim 18 , further comprising:
heating said sample to a temperature ranging from 60° C. to 80° C. for a period ranging from 2 minutes to 5 minutes after said contacting, but before said detecting.
20. The method according to claim 18 , wherein said sample is obtained from a foot, a nail plate, a nail bed, a hair, a dander, a vesicle, and a papula.
21. The method according to claim 18 , wherein said microorganism is fungi.
22. The method according to claim 21 , wherein said fungi is a member of a genus selected from the group consisting of Trichophyton, Candida and Malassezia .