Glucose dehydrogenase and production thereof

1. A soluble pyrroloquinoline quinone glucose dehydrogenase (sPQQGDH) having the amino acid sequence of wild-type Acinetobacter LMD 79:41 (numbering as shown in SEQ ID NO: 2), wherein compared to the amino acid sequence of wild-type Acinetobacter LMD 79:41 (numbering as shown in SEQ ID NO: 2):

a) the following amino acids are exchanged:

i) position 21 has a N;

ii) position 41 has an S;

iii) position 47 has an L;

iv) position 121 has a V or an A;

v) position 149 has an A;

vi) position 213 has an S;

vii) position 244 has an I;

viii) position 320 has a G;

ix) position 391 has an S;

x) position 452 has an S;

xi) position 474 has an R; and

xii) position 480 has a Q; and

b) optionally at least one of the following amino acids are also exchanged:

i) position 16 has an H;

ii) position 18 has an L;

iii) position 20 has an F;

iv) position 40 has a G;

v) position 48 has an I;

vi) position 61 has an A;

vii) position 111 has a T;

viii) position 154 has a D;

ix) position 190 has a D;

x) position 293 has an A;

xi) position 311 has an S;

xii) position 314 has an A;

xiii) position 324 has an L;

xiv) position 333 has an M;

xv) position 339 has an S;

xvi) position 355 has a G;

xvii) position 366 has a D;

xviii) position 417 has an N; or

xix) position 418 has an A.

2. sPQQGDH of claim 1 , wherein compared to wild-type Acinetobacter LMD 79:41 (numbering as shown in SEQ ID NO: 2) at least one of the following amino acids are also exchanged:

i) position 16 has an H;

ii) position 18 has an L;

iii) position 20 has an F;

iv) position 40 has a G;

v) position 48 has an I;

vi) position 61 has an A;

vii) position 111 has a T;

viii) position 154 has a D;

ix) position 190 has a D;

x) position 293 has an A;

xi) position 311 has an S;

xii) position 314 has an A;

xiii) position 324 has an A;

xiv) position 333 has an M;

xv) position 339 has an S;

xvi) position 366 has a D;

xviii) position 417 has an N; or

xix) position 418 has an A.

3. sPQQGDH of SEQ ID NO. 12.

4. Process for preparing the sPQQGDH according to claim 1 , which comprises cultivating a microorganism which expresses the sPQQGDH, and then isolating the sPQQGDH.

5. Process for preparing the sPQQGDH according to claim 2 , which comprises cultivating a microorganism which expresses the sPQQGDH, and then isolating the sPQQGDH.

6. Process for preparing the sPQQGDH according to claim 3 , which comprises cultivating a microorganism which expresses the sPQQGDH, and then isolating the sPQQGDH.

7. Reagent for detection of glucose comprising one or more sPQQGDH enzymes according to claim 1 .

8. Reagent for detection of glucose comprising one or more sPQQGDH enzymes according to claim 2 .

9. Reagent for detection of glucose comprising the sPQQGDH enzyme according to claim 3 .