Novel adult tissue-derived stem cell and use thereof
Subjects of the present invention are to establish a technology for separating, isolating and culturing a stem cell derived from adult tissue while retaining the in vivo properties thereof, and to provide a preventive and/or therapeutic agent for diseases which accompany tissue injury, which comprises a stem cell derived from adult tissue as an active ingredient. In order to solve the subjects, the present invention provides a stem cell derived from adult tissue which is CD45-negative and CXCR4-positive. Also, the present invention provides a preventive and/or therapeutic agent for diseases which accompany tissue injury, which comprises the stem cell as an active ingredient.
1 . A stem cell derived from an adult tissue which is CD45-negative and CXCR4-positive.
2 . The stem cell according to claim 1 , wherein the adult tissue is a tissue selected from the group consisting of bone marrow, skin skeletal muscle, fat tissue and peripheral blood.
3 . The stem cell according to claim 1 , which is obtained by extracting cells from bone marrow by an enzyme treatment, followed by separation using an anti-CD45 antibody, an anti-CD34 antibody and an anti-erythrocyte antibody.
4 . The stem cell according to claim 3 , wherein the enzyme is collagenase.
5 . The stem cell according to claim 1 , wherein the stem cell is pluripotent stem cell.
6 . A method for separating the stem cell according to claim 1 , which comprises extracting cells from bone marrow by an enzyme treatment and separating stem cells using an anti-CD45 antibody, an anti-CD34 antibody and an anti-erythrocyte antibody.
7 . The method according to claim 6 , wherein the enzyme is collagenase.
8 . The method according to claim 6 , wherein the stem cell is a pluripotent stem cell.
9 . A preventive and/or therapeutic agent for diseases which accompany tissue injury, which comprises the stem cell according to claim 1 as an active ingredient.
10 . The preventive and/or therapeutic agent according to claim 9 , wherein the disease which accompanies tissue injury is any one of the neural disease, respiratory organ system disease, cardiovascular disorders, hepatic disease, pancreatic disease, digestive organ system disease, renal disease and skin disease.
11 . A method for growing the stem cell according to claim 1 , which comprises culturing the cell in a medium supplemented with at least one of macrophage colony-stimulating factor (M-CSF) and leukemia inhibitory factor (LIF) on a fibronectin-coated culture dish.
12 . A method for growing the stem cell according to claim 1 , which comprises culturing the cell in a medium supplemented with macrophage colony-stimulating factor (M-CSF), and leukemia inhibitory factor (LIF) on a fibronectin-coated culture dish.
13 . A method for preventing and/or treating diseases which accompany tissue injury, which comprises using the stem cell according to any one of claim 1 .
14 . (canceled)