Compositions and methods including a recombinant human MAB that promotes CNS remyelination
View Patent ↗Antibodies, and particularly human antibodies, are disclosed that demonstrate activity in the treatment of demyelinating diseases as well as other diseases of the central nervous system that are of viral, bacterial or idiopathic origin, including neural dysfunction caused by spinal cord injury. Neuromodulatory agents are set forth that include and comprise a material selected from the group consisting of an antibody capable of binding structures or cells in the central nervous system, a peptide analog, a hapten, active fragments thereof, agonists thereof, mimics thereof, monomers thereof and combinations thereof. Methods are described for treating demyelinating diseases, and diseases of the central nervous system of humans and domestic animals, using polyclonal IgM antibodies and human monoclonal antibodies sHIgm22(LYM 22), sHIgm46(LYM46) ebvHIgM MSI19D10, CB2bG8, AKJR4, CB2iE12, CB2iE7, MSI19E5 and MSI10E10, active fragments thereof and the like. The invention also extends to the use of human antibodies, fragments, peptide derivatives and like materials, and their use in above referenced therapeutic applications, and to pharmaceutical compositions containing them, that may be administered in desirably low doses to treat conditions involving demyelination and to promote remyelination.
1. A method of stimulating remyelination of central nervous system axons in a human with an antibody comprising the heavy chain variable regions CDR1, CDR2 and CDR3 sequences as set out in FIG. 5 and SEQ ID NO:7 and the light chain variable region CDR1, CDR2 and CDR3 sequences as set out in FIG. 6 and SEQ ID NO:9; the antibody selected from the group consisting of: a human monoclonal antibody mAb sHIgM22 (LYM 22), active fragments thereof, or recombinant antibodies derived therefrom; the method comprising:
administering said antibody to a patient in an amount of 1.25 μg antibody/kg body weight to 2.5 μg antibody/kg body weight.
2. A method of claim 1 wherein the administering step comprises:
administering said antibody to a patient at a dose of said antibody/kg body weight selected from the group of: 1.25 μg antibody/kg body weight, 1.3 μg/kg, 1.4 μg/kg, 1.5 μg/kg, 1.6 μg/kg, 1.7 μg/kg, 1.8 μg/kg, 1.9 μg/kg, 2.0 μg/kg, 2.1 μg/kg, 2.2 μg/kg, 2.3 μg/kg, 2.4 μg/kg, and 2.5 μg/kg body weight.
3. A method of stimulating remyelination of central nervous system axons in a mammal with an antibody comprising the heavy chain variable regions CDR1, CDR2 and CDR3 sequences as set out in FIG. 5 and SEQ ID NO:7 and the light chain variable region CDR1, CDR2 and CDR3 sequences as set out in FIG. 6 and SEQ ID NO:9; the antibody selected from the group consisting of: a human monoclonal antibody mAb sHIgM22 (LYM 22), active fragments thereof, or recombinant antibodies derived therefrom; the method comprising:
administering only a single unit dose of a pharmaceutical composition comprising a therapeutically effective amount of an antibody together with pharmaceutically acceptable carrier, vehicle or diluent; and the single unit dose of the antibody is 500 ng.
4. The method of claim 1 , wherein the administering step comprises administration in a single dose.
5. The method of claim 1 , wherein the administering step comprises administration in multiple separated or divided doses.
6. The method of claim 5 , wherein the administering step comprises administration in multiple separated or divided doses daily over the course of days or months.
7. The method claim 1 or claim 3 , wherein the administering step comprises a method of administration selected from the group of: intravenous, intraperitoneal, intrathecal, subcutaneous, sublingual, intramuscular, rectal, respiratory and nasopharyngeal administration.
8. The method of claim 1 or claim 3 , further comprising administering said antibody in combination with a therapeutically effective amount of a steroid.
9. The method of claim 8 , wherein said steroid comprises methylprednisolone.
10. The method of claim 1 or 3 wherein said antibody comprises a heavy chain variable region amino acid sequence as set out in FIG. 5 and SEQ ID NO: 7 and a light chain variable region amino acid sequence as set out in FIG. 6 and SEQ ID NO: 9.
11. A single unit dose of a pharmaceutical composition comprising a therapeutically effective amount of an antibody, comprising:
a single unit dose of an antibody that is 500 ng;
the antibody capable of stimulating remyelination and comprising the heavy chain variable regions CDR1, CDR2 and CDR3 sequences as set out in FIG. 5 and SEQ ID NO:7 and the light chain variable region CDR1, CDR2 and CDR3 sequences as set out in FIG. 6 and SEQ ID NO:9; the antibody selected from the group consisting of: a human monoclonal antibody mAb sHIgM22 (LYM 22), active fragments thereof, or recombinant antibodies derived therefrom; and,
a pharmaceutically acceptable carrier, vehicle or diluent.
12. A therapeutically effective amount of a pharmaceutical composition comprising an antibody, said composition comprising:
an antibody capable of stimulating remyelination and comprising the heavy chain variable regions CDR1, CDR2 and CDR3 sequences as set out in FIG. 5 and SEQ ID NO:7 and the light chain variable region CDR1, CDR2 and CDR3 sequences as set out in FIG. 6 and SEQ ID NO:9; the antibody selected from the group consisting of: a human monoclonal antibody mAb sHIgM22 (LYM 22), active fragments thereof, or recombinant antibodies derived therefrom;
a pharmaceutically acceptable carrier, vehicle or diluent; and,
wherein said pharmaceutical composition comprising an antibody is prepared for delivery in humans in a dose range of 1.25 μg antibody/kg body weight to 2.5 μg antibody/kg body weight.
13. The pharmaceutical composition of claim 12 , wherein said pharmaceutical composition comprising an antibody is prepared for delivery in humans in a dose selected from 1.25 μg antibody/kg body weight, 1.3 μg/kg, 1.4 μg/kg, 1.5 μg/kg, 1.6 μg/kg, 1.7 μg/kg, 1.8 μg/kg, 1.9 μg/kg, 2.0 μg/kg, 2.1 μg/kg, 2.2 μg/kg, 2.3 μg/kg, 2.4 μg/kg, and 2.5 μg antibody/kg body weight.
14. The composition of claim 11 or claim 12 wherein said antibody comprises a heavy chain variable region amino acid sequence as set out in FIG. 5 and SEQ ID NO: 7 and a light chain variable region amino acid sequence as set out in FIG. 6 and SEQ ID NO: 9.