Signal amplification using a synthetic zymogen
View Patent ↗Described herein are zymogens, methods of use for zymogens, and devices that incorporate zymogens. The zymogens include a substrate and an enzyme. The substrate can inhibit the enzyme and is a target for a protein produced by a microorganism. When the substrate is modified by a protein produced by a microorganism, the enzyme is activated. The zymogens can be used to amplify detection assays.
1. A zymogen complex comprising at least two enzymes, each covalently bonded to an exogenous peptide, the exogenous peptide sterically blocking the enzymatic activity of each enzyme, wherein at least one of the enzymes comprises a laccase.
2. A zymogen complex comprising at least two enzymes, each covalently bonded to an exogenous peptide, the exogenous peptide sterically blocking the enzymatic activity of each enzyme, wherein at least one of the enzymes comprises a CotA enzyme.
3. A zymogen complex comprising at least two enzymes, each covalently bonded to an exogenous peptide, the exogenous peptide sterically blocking the enzymatic activity of each enzyme, wherein at least one of the enzymes comprises a CotA mutant.