Method for determining the impact of a multicomponent synthetic product mixture on the biological profile of a disease within a group of living systems and the development of new combinatorial interventions
The invention provides a method for determining the impact of a chemical multicomponent mixture on the biological profile of a disease comprising the steps of: (a) determining a biological profile of the disease by comparing the biological profile of a group of living systems with symptoms of the disease with the biological profile of a reference (or healthy) group of living systems, using a multivariate analysis; (b) determining the impact of a series of samples of one or more synthetic compositions on the biological profile of the disease, in which samples the concentrations of the one or more synthetic compositions differ, using a multivariate analysis; (c) preparing a set of multicomponent synthetic product mixtures which are expected to display a desired impact on the biological profile of the disease on the basis of the information obtained in step (b); and (d) determining the impact of the set of multicomponent mixtures as prepared in step (c) on the biological profile of the disease using multivariate analysis. The invention also provides a method for preparing a medicament, and the use of a multicomponent synthetic product mixture as prepared in step (c) for preparing a synthetic product-based medicament.
1 . A method for determining the impact of a multicomponent synthetic product mixture on the biological profile of a disease within a group of living systems comprising the steps of:
(a) determining a biological profile of the disease by comparing the biological profile of a group of living systems with symptoms of the disease with the biological profile of a reference (or healthy) group of living systems, using a multivariate analysis;
(b) determining the impact of a series of samples of one or more synthetic compositions on the biological profile of the disease, in which samples the concentrations of the one or more synthetic compositions differ, using a multivariate analysis;
(c) preparing a set of multicomponent synthetic product mixtures which are expected to display a desired impact on the biological profile of the disease on the basis of the information obtained in step (b); and
(d) determining the impact of the set of multicomponent mixtures as prepared in step (c) on the biological profile of the disease using multivariate analysis.
2 . A method according to claim 1 , wherein after step (d) from the set of multicomponent synthetic product mixtures prepared in step (c) one or more mixtures are selected in a step (e), which selected mixtures display the desired impact on the biological profile of the disease.
3 . A method according to claim 1 , wherein in step (a) use is made of at least one spectrometric technique, at least one electromigration-based technique or at least one chromatographic technique to determine the profile of the disease.
4 . A method according to claim 1 , wherein in step (b) use is made of at least one spectrometric technique, at least one electromigration-based technique or at least one chromatographic technique to determine the impact of the series of samples of the multicomponent mixture on the biological profile of the disease samples.
5 . A method according to claim 2 , wherein in step (d) use is made of at least one spectrometric technique, at least one electromigration-based technique or at least one chromatographic technique to determine the composition of the samples.
6 . A method according to claim 2 , wherein use is made of two or more spectrometric techniques or electromigration-based techniques.
7 . A method according to claim 6 , wherein use is made of at least a nuclear magnetic resonance technique and a mass spectrometry technique or electromigration-based technique.
8 . A according to claim 1 , wherein the biological profile includes one or more metabolic, genetic and/or proteomic profiles.
9 . A method according to claim 8 , wherein the biological profile includes the metabolic, genetic and proteomic profiles.
10 . A method according to claim 1 , wherein the multicomponent mixture comprises chemical product.
11 . A method according to claim 1 , wherein in step (a) the biological profiles are determined of at least one type of bodyfluid.
12 . A method according to claim 1 , wherein in step (a) the biological profiles are determined of at least one type of tissue.
13 . A method according to claim 12 , wherein in step (a) the biological profiles are determined of at least two different types of bodyfluid.
14 . A method according to claim 1 , wherein in step (a) the biological profiles are determined using one or more of the following biomarkers; genes, transcripts, proteins, metabolites and (trace) elements.
15 . A method according to claim 1 , wherein the number of samples in step (b) is at least 2.
16 . A method according to claim 15 , wherein the number of samples in step (c) ranges from 5-100.
17 . Use of a multicomponent synthetic product mixture as prepared in step (c) as defined in claim 1 for preparing a synthetic product-based medicament.
18 . Use of a multicomponent synthetic product mixture as selected in step (e) as defined in claim 2 for preparing a synthetic product-based medicament:
19 . A medicament comprising a multicomponent synthetic product mixture as prepared in step (c) as defined in claim 1 .
20 . A method for controlling the composition of a multicomponent mixture as selected in step (e) as defined in claim 2 , wherein the concentrations of one or more compositions contained in the mixture are adjusted to ensure that the one or more compositions contained in the mixture have an impact on a biological profile of the disease.
21 . A medicament comprising a multicomponent synthetic product mixture as selected in step (e) as defined in claim 2.