Telechelic homopolyamino acids functionalized with hydrophobic groups, and their applications, especially therapeutic applications
View Patent ↗The invention relates to novel materials based on biodegradable homopolyamino acids and which can be used for the vectorization of (an) active ingredient(s) (AI). The invention also relates to novel pharmaceutical, cosmetic, dietetic or phytosanitaty compositions based on homopolyamino acids. The invention can produce a novel polymer raw material which can be used for the vectoiization of Al that can optimally be: biocompatible, biodegradable, capable of becoming easily associated with a large number of active ingredients or solubilizing them and releasing the active ingredients in vivo. According to the present invention, which primarily relates to linear homopolyamino acids having aspartic or glutamic units and whose attachments can include hydrophobic groups having 8-30 carbon atoms. The homopolymers are amphiphilic and anionic and can easily be transformed at low cost into particles for the vectorization of active ingredients. The particles can form stable aqueous colloidal suspensions.
1. An anionic, linear, amphiphilic homopolyamino acid, wherein its two ends carry hydrophobic groups that are identical to or different from one another; and having one of the general formulae (I), (II), and (III) below:
wherein n+m is defined as the degree of polymerization and varies from 3 to 1000;
wherein n+m is defined as the degree of polymerization and varies from 3 to 1000;
wherein n is defined as the degree of polymerization and varies from 3 to 1000;
and wherein:
R 1 CO—, R 2 CO—, R 1′ , R 2′ , R 1″ and R 2″ independently are a C8 to C30 hydrophobic group;
R 3 is a linear C2 to C6 alkyl group;
R 4 is selected from the group consisting of H, a metal cation, an organic cation, a cation based on amino acid(s), and a cationic polyamino acid;
R 5 is a C2 to C6 alkyl, dialkoxy or diamine group;
A independently is —CH 2 -or —CH 2 —CH2—; and
B is a link formed of a direct covalent bond or an amino acid residue of the formula below:
wherein R is a radical characteristic of a natural amino acid and selected from the group consisting of H (in which case B is a glycine residue), methyl (in which case B is an alanine residue), isobutyl (in which case B is a leucine residue), isopropyl (in which case B is a valine residue) and CH 2 Ph (in which case B is a phenylalanine residue).
2. The homopolyamino acid according to claim 1 , having a molecular weight between 2000 and 100,000 glmol.
3. The pharmaceutical, cosmetic, dietetic or phytosanitary composition comprising at least one homopolyamino acid according to claim 1 .
4. The composition according to claim 3 , said composition comprising at least one active principle.
5. The composition according to claim 4 , wherein the active principle is associated with the homopolyamino acid by one or more bonds other than covalent chemical bonds.
6. The composition according to claim 4 , wherein the active principle is selected from the group consisting of: a protein, a glycoprotein, a protein bonded to one or more polyalkylene glycol chains, a protein bonded to polyethylene glycol (PEG) chains, a polysaccharide, a liposaccharide, an oligonucleotide, a polynucleotide and a peptide.
7. The composition according to claim 4 , wherein the active principle is selected from the group consisting of: a small hydrophobic organic molecule, a hydrophilic organic molecule, and an amphiphilic organic molecule.
8. The composition according to claim 4 , which can be administered by a route selected from the group consisting of: oral, parenteral, nasal, vaginal, ocular, subcutaneous, intravenous, intramuscular, intradermal, intraperitoneal, intracerebral and buccal route.
9. The composition according to claim 4 , wherein said composition form is selected from the group consisting of: a gel, an emulsion, micelles, nanoparticles, microparticles, a powder and a film.
10. The composition according to claim 4 , wherein said composition is a colloidal suspension of nanoparticles, microparticles, or micelles of polyamino acids, in an aqueous phase.
11. The composition according to claim 4 , wherein said composition is in the form of a solution in a biocompatible solvent that is capable of being injected by the subcutaneous or intramuscular route or into a tumor.
12. The homopolyamino acid according to claim 1 , having one of the general formulae (I) or (II), wherein n+m varies from 20 to 300.
13. The homopolyamino acid according to claim 1 , having the general formula (III), wherein n varies from 20 to 300.
14. The homopolyamino acid according to claim 1 , wherein said homopolyamino acid has a molecular weight between 5000 and 40,000 glmol.
15. The homopolyamino acid according to claim 1 , wherein R 4 is a metal cation selected from the group consisting of sodium, potassium, calcium, and magnesium.
16. The homopolyamino acid according to claim 1 , wherein R 4 is an organic cation selected from the group consisting of cations based on amine, cations based on oligoamine, cations based on polyamine, cations based on polyethylenimine, cations based on amino acid(s), and cationic polyamino acids.
17. The homopolyamino acid according to claim 1 , wherein R 4 is a cation based on lysine or arginine.
18. The homopolyamino acid according to claim 1 , wherein R 4 is a cationic polyamino acid selected from the group consisting of polylysine and oligolysine.