Compositions and methods for detecting a non plus-stranded RNA virus
View Patent ↗The invention provides compositions and methods for detecting the presence of SARS-coronavirus, for screening anti-SARS coronavirus agents and vaccines, and for reducing infection with plus-strand RNA viruses such as SARS-coronavirus.
1. A method for detecting a virus that is not a plus-strand RNA virus in a sample, comprising:
a) providing:
i) a sample suspected of comprising at least one virus that is not a plus-strand RNA virus, wherein said not a plus-strand virus is selected from the group consisting of influenza virus, parainfluenza virus, respiratory syncytial virus, and metapneumovirus;
ii) cells susceptible to said virus that is not a plus-strand RNA virus;
iii) an E64D protease inhibitor;
b) contacting said cells of ii) and said sample both in the presence (b1) and absence (b2) of said protease inhibitor to produce contacted cells;
c) wherein, if the level of viral replication in b) in the presence of the protease inhibitor (b1) is not reduced relative to the replication in the absence of the inhibitor (b2), the virus present in the sample of i) is identified as not a plus-strand RNA virus.
2. The method of claim 1 , wherein said influenza virus is chosen from influenza A, influenza B, and influenza C.
3. The method of claim 1 , wherein said parainfluenza virus is chosen from parainfluenza 1, parainfluenza 2, parainfluenza 3, and parainfluenza 4.
4. The method of claim 1 , wherein said protease inhibitor is contained in a composition comprising a cyclodextrin.
5. The method of claim 4 , wherein said cyclodextrin comprises sulfobutyl ether beta-cyclodextrin sodium.