Compositions and method for decreasing the appearance of skin wrinkles
Compositions which include platelet-rich plasma and fibroblast cells are disclosed for the treatment of skin. In particular, administration of platelet-rich plasma in a dermatologically acceptable carrier repeatedly to skin is disclosed to reduce the appearance of wrinkles.
1 . A method of treating human skin, comprising:
determining a site on skin of a human patient in need of treatment; and
topically applying to the site a composition comprising platelet-rich plasma and dermatologically acceptable carrier.
2 . The method of claim 1 , further comprising: rubbing the composition into the skin.
3 . The method of claim 1 , further comprising: allowing the composition to be absorbed into the skin wherein the composition further comprises a skin permeation enhancer.
4 . The method of claim 1 , wherein the site comprises facial skin.
5 . The method of claim 4 , wherein the platelet-rich plasma comprises platelets obtained from the patient being treated.
6 . The method of claim 1 , further comprising: repeating the topical application of the composition to the site about every 6 to 48 hours.
7 . (canceled)
8 . A method, comprising:
extracting blood from a patient;
concentrating platelets from the blood;
combining the platelets with a skin permeation enhancer and a pharmaceutically acceptable carrier for topical application to skin, thereby forming a composition.
9 . The method of claim 8 , wherein the patient to which the composition is applied is the same patient from which the blood is extracted.
10 . The method claim 8 , wherein the carrier is an injectable carrier and is combined with a component chosen from collagen, cultured cells, and mixtures thereof.
11 - 13 . (canceled)
14 . A method of culturing cells, comprising:
isolating cells from a patient;
placing the cells on a medium comprising platelet-rich plasma; and
allowing the cells to proliferate.
15 . The method of claim 14 , wherein the isolated cells are fibroblast cells.
16 . The method of claim 14 , wherein the cells are isolated from the same patient as the platelets of the platelet-rich plasma are obtained.
17 . The method of claim 14 , wherein the isolate cells are stem cells.
18 - 42 . (canceled)
43 . A formulation, comprising:
cells cultured in a medium comprising platelet releasate obtained from the same patient as the cells; and
a pharmaceutically acceptable carrier.
44 . The formulation of claim 43 , wherein the cells are human fibroblast cells and the carrier is an injectable carrier.
45 . (canceled)