Generation of plants with altered oil content
View Patent ↗The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO32.3 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.
1. A transgenic plant comprising a plant transformation vector comprising a nucleotide sequence that encodes a HIO32.3 polypeptide comprising the amino acid sequence of SEQ ID NO:2, or an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2, whereby the transgenic plant has a high oil phenotype relative to a plant of the same species that does not comprise the plant transformation vector.
2. The transgenic plant of claim 1 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
3. A plant part obtained from the plant according to claim 1 .
4. The plant part of claim 3 , which is a seed.
5. A method of producing oil comprising growing the transgenic plant of claim 1 and recovering oil from said plant.
6. A method of producing a high oil phenotype in a plant, said method comprising:
a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a HIO32.3 polypeptide comprising the amino acid sequence of SEQ ID NO:2, or an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2, and
b) growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed, and said transgenic plant exhibits a high oil phenotype relative to a plant of the same species that does not comprise the plant transformation vector.
7. A plant obtained by a method of claim 6 .
8. The plant of claim 7 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
9. The plant of claim 7 , wherein the plant is selected from the group consisting of a plant grown from said progenitor cells, a plant that is the direct progeny of a plant grown from said progenitor cells, and a plant that is the indirect progeny of a plant grown from said progenitor cells.
10. The method of claim 5 , wherein the oil is recovered from a seed of the plant.
11. The transgenic plant of claim 1 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
12. The transgenic plant of claim 1 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
13. The transgenic plant of claim 12 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.
14. The method claim 6 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
15. The method of claim 6 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
16. The method of claim 15 , wherein the nucleotide sequence encodes a HIO32.3 polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.