Process For Producing Astaxanthin-Containing Lipids
The present invention aims to provide a process which, by improving the conventional method of culturing the green alga Haematococcus pluvialis , promotes vegetative cell growth and astaxanthin biosynthesis so markedly as to enable efficient production of astaxanthin-containing lipids from the culture. In order to attain this object, the green alga Haematococcus pluvialis is cultured with an organic nitrogen source being used in a medium at an AN/TN ratio of 65% or less, preferably 43% or less, more preferably 35% or less, to obtain algal bodies in which astaxanthin-containing lipids have been accumulated. If necessary, the astaxanthin-containing lipids may be extracted from the algal bodies and optionally purified.
1 : A process for producing astaxanthin-containing lipids which comprises culturing green alga with an organic nitrogen source being used in a medium at an AN/TN ratio of 65% or less, preferably 43% or less, more preferably 35% or less, to obtain algal bodies in which astaxanthin-containing lipids have been stored.
2 : The process according to claim 1 , further including the steps of extracting the astaxanthin-containing lipids from the algal bodies and optionally purifying the extracted lipids.
3 : The process according to claim 1 , wherein the organic nitrogen source is at least one organic nitrogen source selected from the group consisting of corn steep liquor, soya bean powder, peptone, tripeptone, and polypeptone.
4 : The process according to claim 1 , wherein the organic nitrogen source is used at least 0.1 g/L.
5 : The process according to claim 4 , wherein culture is performed in a reactor under the dark condition.
6 : The process according to claim 5 , wherein culture is performed under aerobic conditions.
7 : The process according to claim 4 , wherein culture is performed in a reactor under the light condition or in an outdoor, closed system.
8 : The process according to claim 5 , wherein astaxanthin is stored at a concentration of at least 10 mg/L of the culture solution or at least 40 pg/cell.