Mounting device for mounting a retainer means for a biological object and corresponding method for laser micro-dissection
View Patent ↗To enable contactless laser microdissection in a closed container ( 10, 11 ), for example in the form of a Petri dish, it is proposed according to the invention to use a holding device which comprises a receiving portion ( 4 ) for arrangement in the container ( 10, 11 ) and a holding portion ( 1 ) for arrangement externally of the container ( 10, 11 ). The receiving portion ( 4 ) serves to receive at least one receiving means ( 9 ), which serves in turn to receive or collect at least one biological specimen obtained by laser microdissection from biological material ( 15 ) located in the container ( 10, 11 ). The holding portion ( 1 ) is coupled to the receiving portion ( 4 ) in contactless manner, for example by means of magnetic coupling, in such a way that, by moving the holding portion ( 1 ), the receiving portion ( 4 ), together with the receiving means ( 9 ) held thereby, may be positioned in the closed container ( 10, 11 ) precisely over the biological specimen in each case to be received.
1. A method for laser microdissection in a container, comprising
the steps of:
arranging a receiving portion, holding at least one receiving element, in the container,
arranging a holding portion externally of the container,
positioning the receiving portion in the container by means of contactless coupling between the holding portion and the receiving portion by moving the holding portion in a direction relative to the container;
holding in the container at least one receiving element for receiving a biological specimen detached by means of laser microdissection from biological material located in the container;
detaching at least one biological specimen by laser microdissection from the biological material located in the container; and
receiving the at least one biological specimen by the at least one receiving element held in the container,
wherein the receiving portion is held in the container by the holding portion by means of the contactless coupling.
2. A method according to claim 1 , wherein
in the step of arranging the holding portion, the holding portion is arranged externally of the container in the vicinity of the receiving portion located in the container.
3. A method according to claim 1 , wherein
in the step of arranging the receiving portion, the receiving portion is arranged on the inside of a cover of the container, and
in the step of arranging the holding portion, the holding portion is arranged on the outside of the cover.
4. A method according to claim 3 , wherein,
after arrangement of the receiving portion on the inside of the cover and of the holding portions on the outside of the cover, the arrangement consisting of the holding portion, the cover and the receiving portion is combined in such a way with a main body of the container that the cover covers the main body and the receiving portion on the inside of the cover is arranged inside the container formed by the main body and the cover.
5. A method according to claim 1 , wherein
the receiving portion is sterilized before the step of arranging the receiving portion is performed.
6. A method according to claim 1 wherein
the holding portion and the receiving portion form a holding device wherein the receiving portion defines at least one hole sized to hold the at least one receiving element and serve as a collecting vessel for collecting a biological specimen recovered using laser microdissection from biological material to be disposed in the container and wherein the holding portion and the receiving portion are coupled in a contactless manner in such a way that the receiving portion is held in the container via the holding portion and the at least one hole of the receiving portion with the at least one receiving element may be positioned at multiple locations within the container by moving the holding portion.
7. A method according to claim 6 , wherein,
to perform the method, the holding device is used to hold the at least one receiving element in the container.
8. A method according to claim 1 , wherein
the method is performed in computer-assisted manner.
9. A method according to claim 1 wherein,
to perform laser microdissection, a combination of a closed container and a holding device that includes the holding portion and the receiving portion, wherein the receiving portion defines at least one hole sized to hold the at least one receiving element and serve as a collecting vessel for collecting a biological specimen recovered using laser microdissection from biological material to be disposed in the container and wherein the holding portion and the receiving portion are coupled in a contactless manner in such a way that the receiving portion is held in the container via the holding portion and the at least one hole of the receiving portion with the at least one receiving element may be positioned at multiple locations within the container by moving the holding portion is used.