Alkaline lipase from Vibrio metschnikovii RH530 N-4-8 and nucleotide sequence encoding the same
View Patent ↗An alkaline lipase isolated from Vibrio metschnikovii RH530 and a polynucleotide sequence encoding the same are provided. The isolated alkaline lipase has an amino acid sequence of SEQ ID NO: 5 and the polynucleotide having a base sequence of SEQ ID NO: 4 encodes the alkaline lipase. The isolated alkaline lipase exhibits an optimal activity at a high pH level, that is, at pH 10˜11, and has very high ratio of residual enzyme activity and high compatibility with a surfactant, so that it can be suitably used as an enzyme for a laundry detergent.
1. An isolated alkaline lipase having an amino acid sequence of SEQ ID NO: 5.
2. A isolated polynucleotide comprising a sequence encoding an amino acid sequence of SEQ ID NO: 5.
3. The polyucleotide of claim 2 , comprising a nucleotide sequence of SEQ ID NO: 4.
4. The polyucleotide of claim 2 , comprising nucleotide sequences of SEQ ID NO: 2 and SEQ ID NO: 4.
5. The polyucleotide of claim 2 , comprising a nucleotide sequence of SEQ ID NO: 1.
6. A recombinant vector comprising the polynucleotide of claim 2 .
7. The recombinant vector of claim 6 , wherein the recombinant vector is pHL1, pHLB29 or pHAAH38.
8. A transformed host cell comprising the recombinant vector of claim 6 .
9. The transformed host cell of claim 8 , wherein the transformed host cell is Escherichia coli.
10. The transformed host cell of claim 9 , wherein the transformed host cell is HB101 (pHL1).
11. A method of producing an alkaline lipase comprising culturing the transformed host cell of claim 8 .
12. A detergent composition comprising the alkaline lipase of claim 1 .
13. The transformed host cell of claim 8 , wherein the recombinant vector is pHL1, pHLB29 or pHAAH38.