Animal protein free media for cultivation of cells
View Patent ↗The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
1. A method of producing an immunogenic composition comprising a virus or a virus antigen, wherein the method comprises:
a) providing a culture of VERO cells that have been grown in an animal protein free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v), wherein at least 90% of the hydrolysates have a molecular weight of less than or equal to 1000 Daltons;
b) infecting the cells with the virus;
c) incubating the infected cells to propagate the virus;
d) harvesting the virus or virus antigen produced; and
e) preparing an immunogenic composition from the harvested virus or virus antigen.
2. The method according to claim 1 , wherein said harvested virus or virus antigen is subjected to purification.
3. The method according to claim 1 , wherein the virus is selected from the group of orthomyxoviruses, paramyxoviruses, reoviruses, picornaviruses, flaviviruses, arenaviruses, herpesviruses, poxviruses, coronaviruses and adenoviruses.
4. The method according to claim 1 , wherein the harvested virus or virus antigen is purified by ion exchange or gel filtration.
5. The method according to claim 1 , wherein the virus is an influenza virus.