Derivatives, conjugates, and antibodies for detecting ecstasy-class analytes
View Patent ↗Compounds including haptens, intermediates, and immunogens that are useful in the production of antibodies specific for the methylenedioxy class of amphetamine derivatives are described. Antibodies specific for the methylenedioxy class of amphetamine derivatives, reagent kits containing antibodies specific for the methylenedioxy class of amphetamine derivatives, methods of producing antibodies specific for the methylenedioxy class of amphetamine derivatives, and methods of detecting analytes including members of the methylenedioxy class of amphetamine derivatives are also described.
1. A compound having the structure
wherein
R 1 is an alkyl group comprising 2–6 carbon atoms,
R 2 is selected from the group consisting of hydrogen and protectng groups,
R 3 is an optionally substituted alkyl group comprising 1–4 carbon atoms, and
Z is L-X-Q wherein L is (CH 2 ) 3 , X is CO, and Q is selected from the group consisting of macromolecular carriers and labels.
2. The compound of claim 1 wherein the macromolecular carrier is selected from the group consisting of proteins, polypeptides, and polysaccharides.
3. The compound of claim 1 wherein the macromolecular carrier is selected from the group consisting of keyhole limpet hemocyanin, bovine serum albtumin, and bovine thyroglobulin.
4. Cell line NEAMP 48.2, ATCC designation PTA-2595, producing a monoclonal antibody binding preferentially to MDEA.
5. A monocional antibody produced from cell line NEAMP 48.2, ATCC designation PTA-5295, the antibody binding preferentially to MDEA.
6. Cell line NEAMP 62. , ATCC designation PTA-5294, producing a monoclonal antibody binding preferentially to MDEA.
7. A monoclonal antibody prodoced from cell line NEAMP 62.1, ATCC designation PTA-5294, the antibody binding preferentially to MDEA.
8. An antibody generated in response to a compound having the structure
wherein
R 1 is an alkyl group comprising 2–6 carbon atoms,
R 2 is selected from the group consisting of hydrogen and protecting groups,
R 3 is an optionally substituted alkyl group comprising 1–4 carbon atoms, and
Z is L-X-Q wherein L is (CH 2 ) 3 , X is CO, and Q is a macromolecular carrier selected from the group consisting of proteins, polypeptides, and polysaccharides.
9. The antibody of claim 8 wherein the protein is selected fron the group consisting of keyhole limpet hemocyanin, bovine serum albumin, and bovine thyroglobulin.
10. The antibody of claim 8 wherein R 3 is ethyl and R 3 is methyl.
11. A method for producing an antibody comprising inoculating a host with an immunogen having the structure
wherein
R 1 is an alkyl group comprising 2–6 carbon atoms,
R 2 is selected from the group consisting of hydrogen and protecting groups,
R 3 is an optionally substituted alkyl group comprising 1–4 carbon atoms, and
Z is L-X-Q wherein L is (CH 2 ) 3 , X is CO, and Q is a macromolecular carrier selected from the group consisting of proteins, polypeptides, and polysaccharides.
12. The method of claim 11 wherein R 1 is ethyl and R 3 is methyl.
13. The method of claim 11 wherein Q is a protein selected from the group consisting of hemocyanins, globulins, and albumins.
14. A method for detecting an analyte in a sample, the analyte comprising an ecstasy drug or an ecstasy drug derivative, comprising:
contacting the sample with the antbody of claim 8 and a conjugate comprising an analyte analog and a detectable label whereby the analyte and the analog compete for binding to the a antibody, and
measureing the labeled conjugate bound to the antibody or measuring the unbound labeled conjugate as a a measure of the analyte in the sample.