IP Library › Granted Patent US 7,060,468
Granted Patent B2
US 7,060,468 · App. 10/625,115 · Granted Jun 13, 2006

Thermostable glucoamylase

View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 7,060,468
App. No.
10/625,115
Granted
Jun 13, 2006
Kind
B2
Abstract

The invention relates to starch conversion processes using glucoamylases derived from Talaromyces emersonii and related glucoamylases.

Claims (15)

1. A process for saccharifying starch, said process comprising contacting starch or partially hydrolyzed starch with a glucoamylase which has an amino acid sequence that has at least 95% identity with SEQ ID NO:7, under conditions that result in saccharification.

2. The process of claim 1 , wherein said glucoamylase is present in the range from 0.05 to 0.5 AGU per gram of dry solids.

3. The process of claim 1 , wherein said starch or partially hydrolyzed starch comprises at least 30 percent by weight of dry solids.

4. The process of claim 1 , further comprising contacting said starch or partially hydrolyzed starch with a debranching enzyme selected from the group of pullulanase and isoamylase.

5. The process of claim 1 , wherein the contacting is conducted at a pH of about 3 to 5.5 and at a temperature of 60–80° C.

6. The process of claim 1 , wherein said glucoamylase is derived from Talaromyces emersonii.

7. The process of claim 1 , further comprising contacting said starch solution with an acidic alpha-amylase.

8. The process of claim 7 , wherein said acidic alpha-amylase is derived from Aspergillus niger.

9. The process of claim 1 , wherein the glucoamylase has an amino acid sequence of SEQ ID NO:7.

10. The process of claim 1 , wherein the glucoamylase has an amino acid sequence that is at least 97% identical with SEQ ID NO:7.

11. The process of claim 1 , wherein the glucoamylase has an amino acid sequence that is at least 99% identical with SEQ ID NO:7.

12. A process for saccharifying liquidified starch solution, which method comprising contacting said starch solution with a glucoamylase which has an amino acid sequence that has at least 95% identity with SEQ ID NO:7.

13. The process of claim 9 , wherein the glucoamylase has an amino acid sequence of SEQ ID NO:7.

14. The process of claim 12 , wherein the glucoamylase has an amino acid sequence that is at least 97% identical with SEQ ID NO:7.

15. The process of claim 12 , wherein the glucoamylase has an amino acid sequence that is at least 99% identical with SEQ ID NO:7.