Agglutination accelerator for immunological measurement
View Patent ↗An object of the present invention is to provide an immunoassay of PSA using an agglutination accelerator, which has an agglutination accelerating effect equal to or stronger than the known agglutination accelerator; hardly generates non-specific turbidity; and hardly generates salting out even in a solution with a high salt concentration. The present invention relates to an immunoassay of a prostate-specific antigen comprising performing an antigen-antibody reaction in the presence of a polymer having a monomer unit derived from a monomer represented by the following general formula [2]: (wherein R 1 -R 3 are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R 4 is an alkylene group; R 5 is an alkylene group optionally having a substituent and optionally having an oxygen atom in a chain; R 6 is a hydrogen atom or a methyl group, and X is an oxygen atom or a —NH— group), and a kit of reagent for an immunoassay comprising a reagent containing an agglutination accelerator for the immunoassay.
1. An immunoassay method of a prostate-specific antigen comprising:
performing an antigen-antibody reaction comprising, contacting a sample with an antibody to a prostate-specific antigen in the presence of a copolymer as an agglutination accelerator, which is dissolved in a reagent, and is obtained by polymerizing a monomer represented by the following general formula [2]:
wherein, R 1 -R 3 are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R 4 is an alkylene group; R 5 is an alkylene group optionally having a substituent and optionally having an oxygen atom in the chain; R 6 is a hydrogen atom or a methyl group; and X is an oxygen atom or a —NH— group, and an aralkyl methacrylate;
measuring a degree of agglutination derived from the antigen-antibody reaction; and
determining an amount of prostate-specific antigen in the sample based on the degree of agglutination derived from the antigen-antibody reaction.
2. The immunoassay method according to claim 1 , wherein the aralkyl methacrylate is benzyl methacrylate.
3. The immunoassay method according to claim 2 , wherein a ratio of the monomer unit derived from the monomer represented by the general formula [2] in the copolymer is 20% or more but less than 100%.
4. The immunoassay method according to claim 3 , wherein a molecular weight of the polymer is 10,000 to 1,000,000.
5. A kit of reagent for immunoassay of a prostate-specific antigen comprising:
a reagent containing a copolymer as an agglutination accelerator, which is dissolved in the reagent, and obtained by polymerizing a monomer represented by the following general formula [2]:
wherein, R 1 -R 3 are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R 4 is an alkylone group; R 5 is an alkylene group optionally having a substituent and optionally having an oxygen atom in the chain; R 6 is a hydrogen atom or a methyl group; and X is an oxygen atom or a —NH— group, and an aralkyl methacrylate; and
a reagent containing an antibody to a prostate-specific antigen or a prostate-specific antigen.
6. The kit according to claim 5 , wherein the antibody to a prostate-specific antigen or the prostate-specific antigen is supported on a carrier.
7. The kit according to claim 6 , wherein the carrier is latex.
8. The kit according to claim 5 , wherein the aralkyl methacrylate is benzyl methacrylate.