Seed-associated promoter sequences
View Patent ↗Plant promoters that exhibit seed-associated expression are described, including bi-directional promoters capable of directing seed-associated expression in either orientation.
1. An isolated nucleic acid molecule comprising a PRU promoter that has seed-associated promoter activity and that comprises, in a 5′ to 3′ direction:
(a) nucleotides 1055–1127 of SEQ ID NO:1 or the reverse complement of nucleotides 142–214 of SEQ ID NO:1, operably linked to
(b) nucleotides 1168–1212 of SEQ ID NO:1 or the reverse complement of nucleotides 58–101 of SEQ ID NO:1.
2. The isolated nucleic acid molecule of claim 1 wherein the PRU promoter additionally comprises nucleotides 854–918 of SEQ ID NO:1 or the reverse complement of nucleotides 365–428 of SEQ ID NO:1 operably linked to the nucleotides set forth in (a) and (b).
3. The isolated nucleic acid molecule of claim 1 wherein the PRU promoter comprises nucleotides 1055–1212 of SEQ ID NO:1.
4. The isolated nucleic acid molecule of claim 3 wherein the PRU promoter comprises nucleotides 854–1212 of SEQ ID NO:1.
5. The isolated nucleic acid molecule of claim 4 wherein the PRU promoter comprises SEQ ID NO:1.
6. The isolated nucleic acid molecule of claim 1 wherein the PRU promoter comprises the reverse complement of nucleotides 58–214 of SEQ ID NO:1.
7. The isolated nucleic acid molecule of claim 6 wherein the PRU promoter comprises the reverse complement of nucleotides 58–428 of SEQ ID NO:1.
8. The isolated nucleic acid molecule of claim 7 wherein the PRU promoter comprises the reverse complement of SEQ ID NO:1.
9. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is a plant expression vector.
10. The plant expression vector of claim 9 , wherein the PRU promoter is operably linked to a heterologous protein-encoding sequence.
11. The plant expression vector of claim 9 comprising a first heterologous protein-encoding sequence in the antisense direction, the PRU promoter, and a second heterologous protein-encoding sequence in the sense direction, wherein the vector is double-stranded, and wherein the PRU promoter directs seed-associated expression of both the first and the second heterologous protein-encoding sequences.
12. A transgenic plant cell comprising a plant expression vector of claim 9 in its genome.
13. The plant cell of claim 12 , wherein the plant cell is from a plant belonging to the Prunus genus.
14. The plant cell of claim 13 , wherein the plant is selected from the group consisting of cherry, almond, peach, apricot, and plum.
15. The plant cell of claim 12 , wherein the plant is from the Arabidopsis genus.
16. A method for producing a transgenic plant that exhibits seed-associated expression of a heterologous protein-encoding sequence, comprising:
transforming progenitor cells of the plant with the plant expression vector of claim 10 , and
growing the transformed progenitor cells to produce a transgenic plant that exhibits seed-associated expression of the heterologous protein-encoding sequence.
17. A transgenic plant comprising the cell of claim 12 .
18. The plant of claim 17 , wherein the plant belongs to the Prunus genus.
19. The plant of claim 18 , wherein the plant is selected from the group consisting of cherry, almond, peach, apricot, and plum.
20. The plant of claim 17 , wherein the plant belongs to the Arabidopsis genus.
21. A plant part obtained from a plant according to claim 17 .
22. The plant part of claim 21 , wherein the plant part is a seed.
23. The nucleic acid of claim 1 , wherein the PRU promoter comprises, in a 5′ to 3′ direction, nucleotides 1055–1127 of SEQ ID NO:1 operably linked to nucleotides 1168–1212 of SEQ ID NO:1.
24. The nucleic acid of claim 23 , wherein the PRU promoter further comprises nucleotides 854–918 of SEQ ID NO:1.
25. The nucleic acid of claim 1 , wherein the PRU promoter comprises, in a 5′ to 3′ direction, the reverse complement of nucleotides 142–214 of SEQ ID NO:1 operably linked to the reverse complement of nucleotides 58–101 of SEQ ID NO:1.
26. The nucleic acid of claim 1 , wherein the PRU promoter further comprises the reverse complement of nucleotides 365–428 of SEQ ID NO:1.