Compounds for targeting hepatocytes in vivo
View Patent ↗We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
1. A composition for targeting hepatocytes comprising: a T7 ligand covalently attached to a compound, wherein,
a) said T7 ligand is selected from the group consisting of: T7 p17 protein, modified T7 p17 protein, T7 p17 rod domain, T7 p17 coiled coil domain, SEQ ID NO: 1, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, and
b) said compound is selected from the group consisting of: drug, component of a drug or polynucleotide complex and polynucleotide.
2. The composition of claim 1 wherein the drug comprises interferon.
3. The composition of claim 1 wherein the complex consists of a liposome.
4. The composition of claim 1 wherein the complex consists of a polyplex.
5. The composition of claim 1 wherein the complex consists of a lipopolyplex.
6. The composition of claim 1 wherein the complex comprises a polynucleotide.
7. The composition of claim 6 wherein the polynucleotide consists of an expression cassette.
8. The composition of claim 6 wherein the polynucleotide consists of an RNA.
9. The composition of claim 1 wherein the polynucleotide consists of an RNA function inhibitor.
10. The composition of claim 1 wherein the T7 ligand contains a functional group.
11. The composition of claim 10 wherein the functional group consists of a thiol.
12. The composition of claim 11 wherein the thiol consists of a cysteine.
13. The composition of claim 10 wherein the functional group consists of biotin.
14. The composition of claim 10 wherein the functional group consists of streptavidin.
15. The composition of claim 1 wherein the T7 ligand is attached to the compound via a linker.
16. The composition of claim 15 wherein the linker consists of a polyethylene glycol.
17. The composition of claim 1 wherein the T7 ligand consists of: T7 ligand-cysteine-PDP-streptavidin.
18. The composition of claim 1 wherein the T7 ligand consists of T7 ligand-PEG-biotin.
19. A pharmaceutical preparation comprising: a compound selected from the group consisting of: drug, component of a drug or polynucleotide complex, and polynucleotide covalently attached to a T7 ligand selected from the group consisting of: T7 p17 protein, modified T7 p17 protein, T7 p17 rod domain, T7 p17 coiled coil domain, SEQ ID NO: 1, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24.
20. A composition for targeting hepatocytes in vivo comprising: a T7 ligand covalently attached to a compound, wherein
a) said T7 ligand is selected from the group consisting of: T7 p17 protein, modified T7 p17 protein, T7 p17 rod domain, T7 p17 coiled coil domain, SEQ ID NO: 1, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, and
b) said compound is selected from the group consisting of: drug, component of a drug or polynucleotide complex and polynucleotide.