Multi-column separation devices and methods
View Patent ↗Chromatographic separation devices include multiple batch-processed columns joined by a body structure and adapted to perform parallel analyses. Both slurry-packed and monolithic column embodiments are provided. One or more liquid-permeable frits of various types may be used to retain stationary phase material within columns. A fluidic distribution network may be used to distribute stationary phase material and/or mobile phase solvents to multiple columns. Separation devices, including microfluidic embodiments, may be fabricated with various materials including polymers. Multi-column fabrication and separation methods are provided.
1. A device for performing a plurality of simultaneous liquid chromatographic separations, the device comprising:
a plurality of batch-processed separation columns containing packed stationary phase material;
at least one liquid-permeable frit adapted to retain the packed stationary phase material within the plurality of separation columns, the at least one liquid-permeable frit being compositionally different from the packed stationary phase material; and
a body structure connecting the plurality of separation columns;
wherein the device is adapted to ensure that each column of the plurality of batch-processed separation columns performs similarly in a parallel liquid chromatography system.
2. The device of claim 1 , further comprising a plurality of sample inlet ports, wherein each separation column of the plurality of separation columns has an associated sample inlet port of the plurality of sample inlet ports.
3. The device of claim 2 wherein each separation column has a first end and a second end, and each sample inlet port of the plurality of sample inlet ports is disposed between the first end and the second end of its associated separation column.
4. The device of claim 2 , further comprising a plurality of sample injection valves in fluid communication with the plurality of sample inlet ports.
5. The device of claim 1 , further comprising:
a common mobile phase source; and
a fluidic distribution network disposed between the common mobile phase source and the plurality of separation columns, wherein each separation column of the plurality of separation columns is in fluid communication with the common mobile phase source through the fluidic distribution network.
6. The device of claim 5 wherein the at least one liquid-permeable frit comprises a first frit disposed between each separation column of the plurality of separation columns and the fluidic distribution network, and a second frit disposed downstream of each separation column of the plurality of separation columns.
7. The device of claim 5 wherein the fluidic distribution network is contained within the body structure.
8. The device of claim 7 wherein the fluidic distribution network is microfluidic.
9. The device of claim 1 , further comprising a plurality of detection regions permitting detection of at least one property of a substance received from the plurality of separation columns, wherein each separation column of the plurality of separation columns has an associated detection region of the plurality of detection regions.
10. The device of claim 1 wherein each column of the plurality of columns has a characteristic fluidic impedance, and the fluidic impedance of each column of the plurality of columns varies by less than about five percent.
11. The device of claim 1 wherein each column of the plurality of columns has a characteristic fluidic impedance, and the fluidic impedance of each column of the plurality of columns varies by less than about two percent.
12. The device of claim 1 wherein the body structure comprises at least one surface adapted to threadlessly engage a mating surface of an external clamping apparatus.
13. The device of claim 1 , further comprising at least one threaded endfitting.
14. The device of claim 1 wherein each separation column of the plurality of separation columns is contained within a substantially cylindrical tube.
15. The device of claim 1 wherein each separation column of the plurality of separation columns is microfluidic.
16. The device of claim 1 wherein each separation column of the plurality of separation columns has a substantially rectangular cross-section.
17. The device of claim 1 wherein the liquid-permeable frit comprises a microporous polymeric material.
18. The device of claim 1 wherein the liquid-permeable frit comprises a metallic material.
19. The device of claim 1 wherein the liquid-permeable frit comprises a sintered material.
20. The device of claim 1 wherein the plurality of columns, the at least one frit, and the body structure are integrated into a disposable cartridge.
21. The device of claim 1 wherein the packed stationary phase material comprises packed particles, and the particles comprise silica, alumina, zirconium, or polymeric materials.
22. The device of claim 21 wherein the packed particles are unsintered.
23. The device of claim 21 wherein at least some of the packed particles include surface functional groups.
24. The device of claim 23 wherein the surface functional groups are selected from the group consisting of: alkyl, cyano, amino, nitro, hydroxy, phenyl, phenyl-hexyl, and sulfonic acid.
25. The device of claim 1 wherein the body structure comprises a polymeric material.
26. The device of claim 1 wherein the body structure comprises a plurality of substantially planar device layers.
27. The device of claim 26 wherein the plurality of substantially planar device layers includes at least one stencil layer having at least one channel defined through the entire thickness of the at least one stencil layer.
28. The device of claim 26 wherein the plurality of substantially planar device layers comprise polymeric materials and are adhesivelessly bound together.
29. The device of claim 1 wherein each separation column of the plurality of separation columns comprises a channel bounding material that is irreversibly affixed to the body structure.
30. The device of claim 1 wherein the device is adapted to perform pressure-driven chromatographic separation.
31. The device of claim 1 wherein the device is adapted to operate at an internal pressure of at least about 100 psi.
32. A fluidic system comprising:
the device of claim 1 ;
a common mobile phase source;
a fluidic distribution network disposed between the common mobile phase source and the plurality of separation columns, wherein each separation column of the plurality of separation columns is in fluid communication with the common mobile phase source through the fluidic distribution network;
a plurality of parallel fluid flow paths disposed downstream of the plurality of separation columns, each parallel fluid flow path being in fluid communication with a different separation column of the plurality of separation columns; and
a plurality of detection regions disposed downstream of the plurality of parallel fluid flow paths and permitting detection of at least one property of a substance received from the plurality of separation columns;
wherein the fluidic distribution network defines a first volume, the plurality of parallel fluid flow paths defines a second volume, the plurality of separation columns defines a third volume, and the third volume is substantially greater than each of the first volume and the second volume.
33. The system of claim 32 wherein the third volume is at least about three times greater than each of the first volume and the second volume.
34. The device of claim 32 wherein the third volume is at least about six times greater than each of the first volume and the second volume.