IP Library Granted Patent US 7,223,986
Granted Patent B2
US 7,223,986 · App. 10/650,062 · Granted May 29, 2007

Laser scanning microscope

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Quick Facts
Patent No.
US 7,223,986
App. No.
10/650,062
Granted
May 29, 2007
Kind
B2
Abstract

There is disclosed a laser scanning microscope including a first optical scanning system which scans a first laser light for observing a sample on the sample, a first light branch device which branches the light from the sample from an optical path of the first laser light, a photodetector which detects the light from the sample, separated by the first light branch device, a second optical scanning system which irradiates a specific portion on the sample with a second laser light for stimulating or operating the sample, and a wavelength selection device which is disposed between the first light branch device and photodetector and which includes a first function of transmitting a desired observation light and a second function of limiting transmission of the second laser light.

Claims (47)

1. A laser scanning microscope comprising:

a first optical scanning system which scans a first laser light for observing a sample on the sample;

a first light branch device which separates a light from the sample from an optical path of the first laser light;

at least one photodetector which detects the light from the sample separated by the first light branch device;

a second optical scanning system which irradiates a specific portion on the sample with a second laser light for stimulating or operating the sample; and

a wavelength selection device which is disposed between the first light branch device and the photodetector and which has a first function of transmitting a desired observation light and a second function of limiting transmission of the second laser light;

wherein the second optical scanning system is attachable and detachable with respect to a main body of the laser scanning microscope that includes the first optical scanning system.

2. A laser scanning microscope comprising:

a first optical scanning system which scans a first laser light for observing a sample on the sample;

a first light branch device which separates a light from the sample from an optical path of the first laser light;

at least one photodetector which detects the light from the sample separated by the first light branch device;

a second optical scanning system which irradiates a specific portion on the sample with a second laser light for stimulating or operating the sample; and

a wavelength selection device which is disposed between the first light branch device and the photodetector and which has a first function of transmitting a desired observation light and a second function of limiting transmission of the second laser light;

wherein the wavelength selection device comprises an interference filter.

3. The laser scanning microscope according to claim 1 , wherein the desired observation light is a fluorescence excited by the first laser light.

4. The laser scanning microscope according to claim 1 , wherein a transmittance of the second laser light by the wavelength selection device is not more than 0.01%.

5. The laser scanning microscope according to claim 1 , wherein the wavelength selection device comprises:

at least one first interference filter which performs the first function; and

at least one second interference filter which performs the second function.

6. The laser scanning microscope according to claim 5 , further comprising:

a wavelength change section which changes a wavelength of the second laser light; and

a filter change section which changes the second interference filter in accordance with the wavelength of the second laser light.

7. The laser scanning microscope according to claim 5 , wherein:

the at least one photodetector comprises a plurality of photodetectors and the at least one first interference filter comprises a plurality of first interference filters;

a second light branch device which splits the light from the sample toward the photodetectors is disposed between the first light branch device and the photodetectors;

the second interference filter is disposed between the first and second light branch devices; and

the first interference filters are disposed between the respective photodetectors and the second light branch device.

8. A laser scanning microscope comprising:

a first optical scanning system which scans a first laser light for observing a sample on the sample;

a first light branch device which separates a light from the sample from an optical path of the first laser light;

at least one photodetector which detects the light from the sample separated by the first light branch device;

a second optical scanning system which irradiates a specific portion on the sample with a second laser light for stimulating or operating the sample; and

a wavelength selection device which is disposed between the first light branch device and the photodetector and which has a first function of transmitting a desired observation light and a second function of limiting transmission of the second laser light;

wherein the wavelength selection device comprises:

at least one first interference filter which performs the first function; and

at least one second interference filter which performs the second function.

9. The laser scanning microscope according to claim 8 , wherein:

the at least one photodetector comprises a plurality of photodetectors and the at least one first interference filter comprises a plurality of first interference filters;

a second light branch device which splits the light from the sample toward the photodetectors is disposed between the first light branch device and the photodetectors;

the second interference filter is disposed between the first and second light branch devices; and

the first interference filters are disposed between the respective photodetectors and the second light branch device.

10. The laser scanning microscope according to claim 8 , further comprising:

a wavelength change section which changes a wavelength of the second laser light; and

a filter change section which changes the second interference filter in accordance with the wavelength of the second laser light.

11. The laser scanning microscope according to claim 8 , wherein a transmittance of the second laser light by the wavelength selection device is not more than 0.01%.

12. The laser scanning microscope according to claim 8 , wherein the second laser light is an ultraviolet or infrared light.

13. The laser scanning microscope according to claim 8 , wherein the desired observation light is a fluorescence excited by the first laser light.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 25, 2023
From: OLYMPUS CORPORATION
To: EVIDENT CORPORATION
Reel/Frame 062492/0267 →
CHANGE OF ADDRESS Recorded Jun 27, 2016
From: OLYMPUS CORPORATION
To: OLYMPUS CORPORATION
Reel/Frame 039344/0502 →