IP Library › Granted Patent US 6,884,620
Granted Patent B2
US 6,884,620 · App. 10/652,703 · Granted Apr 26, 2005

Humanized polynucleotide sequence encoding Renilla mulleri green fluorescent protein

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Quick Facts
Patent No.
US 6,884,620
App. No.
10/652,703
Granted
Apr 26, 2005
Kind
B2
Abstract

The present invention provides a polynucleotide encoding a green fluorescent protein from Renilla mulleri comprising a humanized sequence which permits enhanced expression of the encoded polypeptide in mammalian cells.

Claims (4)

1. A humanized polynucleotide encoding R. mulleri GFP comprising the sequence of SEQ ID NO: 1, wherein at least one codon among the 179 codons that can be humanized as shown in FIG. 4 is not humanized.

2. A humanized polynucleotide encoding R. mulleri GFP comprising the sequence of SEQ ID NO: 1, wherein at least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, or twenty codons among the 179 codons that can be humanized as shown in FIG. 4 are not humanized.

3. A recombinant vector comprising a polynucleotide of claim 1 .

4. A cell containing a recombinant vector of claim 3 .