Method for collecting a desired blood component and performing a photopheresis treatment
View Patent ↗An improved method for separating whole blood into components and collecting a desired blood component. The method allows a desired blood component to be subjected to centrifugal forces within a separator for prolonged periods of time, yielding a cleaner cut and higher yield of the desired blood component. Whole blood is drawn from a source and pumped into a separator, the undesired blood components are removed from the separator at rates so as to build up the desired blood component in the separator. The desired blood component is only removed after a predetermined amount of the desired blood component has built up in the separator. It is preferred that the desired blood component be buffy coat and that the method be used to perform photopheresis treatments. In another aspect, the invention is a method of performing a full photopheresis treatment to treat diseases in a reduced time, preferably less than about 70 minutes, and more preferably less than about 45 minutes.
1. A method of performing a photopheresis treatment for diseases on a patient comprising: drawing whole blood from a source; adding an anticoagulant fluid to the whole blood in a predetermined ratio to form a mixture of whole blood and anticoagulant fluid; separating the mixture of whole blood and anticoagulant into a plurality of blood components according to density; mixing a photoactivation chemical with at least one of the blood components to form a mixture of the photoactivation chemical and the at least one blood component; irradiating the combination of the at least one blood component and photoactivation chemical; and returning the irradiated combination to a patient; wherein blood components are pumped and cycled at a controlled rate so that the entire photopheresis treatment is completed in less than about 70 minutes.
2. The method of claim 1 wherein the entire photopheresis treatment is completed in less than about 45 minutes.
3. The method of claim 1 wherein the at least one blood component is buffy coat, a leukocyte, or platelets.
4. The method of claim 1 wherein the at least one blood component is buffy coat.
5. The method of claim 4 wherein the step of separating the mixture of whole blood and anticoagulant fluid comprises: providing a separator having an inlet, a first outlet, and a second outlet; pumping the mixture of whole blood and anticoagulant fluid into the separator via the inlet at a selected inlet rate; and withdrawing plasma and red blood cells from the separator while continuing to pump the mixture of whole blood and anticoagulant fluid into the separator, the plasma and red blood cells being withdrawn at rates so as to build up buffy coat in the separator, the plasma being withdrawn via the first outlet and the red blood cells being withdrawn via the second outlet; and upon a predetermined amount of buffy coat building up in the separator, collecting the buffy coat from the separator.
6. The method of claim 5 wherein the step of collecting the buffy coat from the separator comprises discontinuing the withdrawal of red blood cells from the second outlet, thereby causing the red blood cells to push the buffy coat out of the separator via the first outlet; and discontinuing the collecting of buffy coat when red blood cells are detected in an outlet line fluidly connected to the first outlet.
7. The method of claim 5 wherein the step of withdrawing plasma and red blood cells from the separator comprises: withdrawing only the plasma from the separator via the first outlet until a predetermined amount of red blood cells are detected in the separator; and upon the predetermined amount of red blood cells being detected in the separator, withdrawing red blood cells from the separator at a rate so as to maintain the amount of red blood cells present in the separator at approximately the predetermined amount.
8. The method of claim 5 further comprising irradiating the collected buffy coat within an irradiation chamber until a predetermined amount of energy has been transferred to the collected buffy coat, the predetermined amount of energy being sufficient to induce apoptosis.
9. The method of claim 1 wherein the source is the patient, the photopheresis treatment being performed in a closed-loop system.