Making a matrix of random encoded arrays
View Patent ↗Disclosed is an apparatus and method of maintaining chips or arrays of particles, or arrays of particles deposited on chip surfaces, in a designated area on a substrate surface. This is accomplished by having hydrophilic chips or arrays of particles, and a substrate which has a series of hydrophilic regions designed to accommodate the chips/arrays of particles, surrounded by hydrophobic regions. In the presence of aqueous solution, the chips or arrays of particles are held in the designated regions.
1. A process of forming a plurality of random arrays of encoded particles on a substrate, wherein each of the arrays are formed by random placement of encoded particles, wherein differently encoded particles have a different label, comprising:
(i) placing and confining a group of differently encoded particles into one or more sites on a substrate having a plurality of sites, so as to form a first confined group of particles occupying particular sites;
(ii) recording the positions of the encoded particles within said confined group;
(iii) placing and confining an additional group of differently encoded particles into unoccupied sites on the substrate so as to form an additional group of confined particles occupying particular sites;
(iv) recording the positions of particles within said additional confined group; and
repeating steps (iii) to (iv) several times so as to form a plurality of a random encoded arrays of particles.
2. The substrate of claim 1 wherein the substrate comprises silicon or doped silicon.
3. The substrate of claim 1 wherein differently encoded particles have different biological reagents bound thereto.
4. The process of claim 1 wherein the arrangement of the encoded particles in the sites is random.
5. The process of claim 1 further comprising the step of affixing thc particles onto the substrate.
6. The process of claim 1 wherein the encoded particles are placed and confined in the sites by first placing the encoded particles into an electrolyte solution on a substrate and then applying an electric field so as to translocate and confine the encoded particles into the sites.
7. The process of claim 1 wherein the substrate is a planar electrode, and another planar electrode is provided, and the substrate and the planar electrode face each other but lie in different planes and the electrolyte solution is located between them, and they are used to apply the electric fields, and wherein at least one of the electrodes is light sensitive and an illumination pattern projected thereon is used, in conjunction with the electric field, to translocate the encoded particles to the illuminated areas in the pattern.
8. The process of claim 1 wherein the electric field and the illumination pattern are controlled by a computer and a user interface system to confine, translocate, and merge encoded particles as desired.