Method for assaying whole blood
View Patent ↗In a method for measuring an analyte, which comprises a reaction step of forming a reaction system including a sample containing whole blood, a first substance carried by a solid carrier and specifically binding to an analyte contained in the sample and a second substance specifically binding to the analyte and allowing the analyte to react with the first and second substances and a measurement step of measuring the formed reaction product, (1) the reaction step is performed in a state that blood cells are not disrupted, and (2) at least the reaction step is performed in the presence of a sufficient amount of a detergent that does not cause hemolysis, does not inhibit reactions of the analyte with the first and second substances specifically binding to the analyte and can prevent influence on the reaction system of a component existing in the reaction system.
1. A method for measuring quantitatively or qualitatively an analyte in a whole blood sample, comprising:
forming a reaction system by mixing the whole blood sample with a whole blood treatment solution comprising detergent of the type that does not cause hemolysis, and adding to the mixture of the whole blood sample and the whole blood treatment solution a first substance which is immobilized on a solid carrier and specifically binds to an analyte contained in the whole blood sample and a second substance which specifically binds to the analyte to allow the analyte to react with the first and second substances to form a complex of first substance-analyte-second substance,
separating the complex, and
detecting the complex to measure quantitatively or qualitatively the analyte in the complex,
wherein said reaction system comprises the detergent in a concentration range of 0.5 to 5% so that hemolysis is prevented.
2. The method according to claim 1 , wherein the detergent is selected from the group consisting of polyoxyethylene sorbitan ester type detergents and sulfobetaine type detergents.
3. The method according to claim 1 , wherein the ratio of the whole blood sample and the whole blood treatment solution is in the range of 99:1 to 5:95.
4. The method according to claim 1 , wherein the reaction system is formed by mixing the whole blood sample with a whole blood treatment solution comprising detergent, then adding the first substance to the mixture of the whole blood sample and the whole blood treatment solution, and then adding the second substance to the mixture of the whole blood sample, the whole blood treatment solution, and the first substance.
5. The method according to claim 1 , wherein the second substance is labeled with a labeling substance.
6. The method according to claim 1 , wherein the first and second substances which specifically bind to the analyte are an antigen or an antibody.
7. A reagent kit for measuring an analyte in a whole blood sample, which comprises a first substance which is immobilized on a solid carrier and specifically binds to the analyte, a second substance which specifically binds to the analyte, and a whole blood treatment solution which comprises detergent of the type that does not cause hemolysis,
wherein said reaction system comprises the detergent in a concentration range of 0.5 to 5% so that hemolysis is prevented.