IP Library › Granted Patent US 7,270,957
Granted Patent B2
US 7,270,957 · App. 10/659,980 · Granted Sep 18, 2007

Method for detecting ammonia-oxidizing bacteria

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Quick Facts
Patent No.
US 7,270,957
App. No.
10/659,980
Granted
Sep 18, 2007
Kind
B2
Abstract

Bacteria that are capable of ammonia oxidation. Particular bacteria of the present invention are tolerant of freshwater environments, saltwater environments or both. Furthermore, in various embodiments, various bacteria of the present invention are capable of surviving a freeze-drying process, and may remain viable thereafter. Methods for detecting the bacteria of the present invention are also provided. Such methods may be effected by any conventional methology, such as with a DNA chip.

Claims (12)

1. A method for detecting and determining the quantity of bacteria that oxidize ammonia to nitrite in a medium, wherein the 16S rDNA of the bacteria includes a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2, comprising:

providing a detectably labeled probe comprising a nucleotide sequence of SEQ ID NO:5;

isolating total DNA from the medium;

exposing the isolated total DNA to the detectably labeled probe under conditions under which the probe hybridizes to only the nucleic acid of the 16S rDNA having the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, thereby forming a hybridized probe; and

detecting and measuring the amount of the hybridized probe,

wherein the presence of hybridized probe is indicative of the presence of bacteria that oxidize ammonia to nitrite and the amount of hybridized probe is indicative of the quantity of said bacteria that oxidize ammonia to nitrite in said medium.

2. The method of claim 1 , wherein the medium is selected from the group consisting of aquarium water, freshwater, saltwater and wastewater.

3. The method of claim 1 , wherein the medium includes a material selected from the group consisting of aquarium gravel, filter sponges, filter floss and plastic filter media.

4. The method of claim 3 , wherein the total DNA is isolated from the material.

5. The method of claim 1 , wherein providing a detectably labeled probe further comprises including the detectably labeled probe on a DNA chip.

6. The method of claim 1 , wherein the method for detecting and determining the quantity of bacteria that oxidize ammonia to nitrite in a medium is an automated process.

7. The method of claim 6 , wherein the automated process is selected from the group consisting of DNA microarray, biosensor, bioprobe, capillary electrophoresis and real-time PCR.

Assignments (9)
NOTICE OF SUCCESSOR AGENT AND ASSIGNMENT OF SECURITY INTEREST (INTELLECTUAL PROPERTY) REEL/FRAME 036131/0272 Recorded Jun 5, 2018
From: DEUTSCHE BANK AG NEW YORK BRANCH
To: ROYAL BANK OF CANADA
Reel/Frame 046301/0425 →
SECURITY INTEREST Recorded Jul 20, 2015
From: SPECTRUM BRANDS, INC.
To: DEUTSCHE BANK AG NEW YORK BRANCH, AS COLLATERAL AGENT
Reel/Frame 036131/0272 →
RELEASE OF SECURITY INTEREST Recorded Jul 14, 2015
From: WELLS FARGO BANK, NATIONAL ASSOCIATION, AS COLLATERAL TRUSTEE
To: APPLICA CONSUMER PRODUCTS, INC.; APPLICA CONSUMER PRODUCTS, INC., A CORP. OF FLORIDA; KWIKSET CORPORATION; LIQUID HOLDING COMPANY, INC.; NATIONAL MANUFACTURING CO.; PRICE PFISTER, INC.; ROV HOLDING, INC., A CORP. OF DELAWARE; ROVCAL, INC.; ROVCAL, INC., A CORP. OF CALIFORNIA; RUSSELL HOBBS, INC., A CORP. OF DELAWARE; SALIX ANIMAL HEALTH, LLC; SEED RESOURCES, L.L.C.; SPECTRUM BRANDS, INC.; SPECTRUM BRANDS, INC. AS SUCCESSOR IN INTEREST TO ROVCAL, INC.; SPECTRUM BRANDS, INC., A CORP. OF DELAWARE; TELL MANUFACTURING, INC.; TETRA HOLDING (US), INC., A CORP. OF DELAWARE; TETRA HOLDINGS (US), INC.; UNITED INDUSTRIES CORPORATION; UNITED INDUSTRIES CORPORATION AS SUCCESSOR IN INTEREST TO LIQUID HOLDING COMPANY, INC.; UNITED PET GROUP, INC.; UNITED PET GROUP, INC., A CORP. OF DELAWARE
Reel/Frame 036102/0001 →
RELEASE OF SECURITY INTEREST Recorded Jul 2, 2015
From: BANK OF AMERICA, N.A., AS AGENT
To: RUSSELL HOBBS, INC.; APPLICA CONSUMER PRODUCTS, INC.; TOASTMASTER INC.; SPECTRUM BRANDS, INC.; ROVCAL, INC.; TETRA HOLDING (US), INC.; UNITED PET GROUP, INC.; SEED RESOURCES, L.L.C.; PRICE PFISTER, INC.; KWIKSET CORPORATION; NATIONAL MANUFACTURING CO.; LIQUID HOLDING COMPANY, INC.; TELL MANUFACTURING, INC.; SALIX ANIMAL HEALTH, LLC
Reel/Frame 036052/0845 →
SECURITY INTEREST Recorded May 7, 2015
From: SPECTRUM BRANDS, INC.; UNITED INDUSTRIES CORPORATION; TELL MANUFACTURING, INC.; SALIX ANIMAL HEALTH, LLC
To: WELLS FARGO BANK, NATIONAL ASSOCIATION
Reel/Frame 035614/0356 →
SECURITY AGREEMENT Recorded Aug 11, 2010
From: RUSSELL HOBBS, INC.; APPLICA CONSUMER PRODUCTS, INC.; TOASTMASTER INC.; SPECTRUM BRANDS, INC.; ROVCAL, INC.; UNITED PET GROUP, INC.; UNITED INDUSTRIES CORPORATION
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 024823/0177 →
SECURITY AGREEMENT Recorded Jul 21, 2010
From: RUSSELL HOBBS, INC., A CORP. OF DELAWARE; SPECTRUM BRANDS, INC., A CORP. OF DELAWARE; ROV HOLDING, INC., A CORP. OF DELAWARE; TETRA HOLDING (US), INC., A CORP. OF DELAWARE; UNITED PET GROUP, INC., A CORP. OF DELAWARE; APPLICA CONSUMER PRODUCTS, INC., A CORP. OF FLORIDA; ROVCAL, INC., A CORP. OF CALIFORNIA
To: WELLS FARGO BANK, NATIONAL ASSOCIATION
Reel/Frame 024741/0295 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 14, 2009
From: GOLDMAN SACHS CREDIT PARTNERS L.P.
To: THE BANK OF NEW YORK MELLON, AS COLLATERAL AGENT
Reel/Frame 022951/0236 →
SECURITY AGREEMENT Recorded Jun 27, 2007
From: AQUARIA, INC.; AQUARIUM SYSTEMS, INC.; UNITED PET GROUP, INC.; SPECTRUM BRANDS, INC. (FORMERLY KNOWN AS RAYOVAC CORPORATION); ROVCAL, INC.; SOUTHERN CALIFORNIA FOAM, INC.; TETRA HOLDING (US), INC.; UNITED INDUSTRIES CORPORATION
To: GOLDMAN SACHS CREDIT PARTNERS L.P., AS COLLATERAL AGENT
Reel/Frame 019477/0974 →