RNA-mediated gene modulation
View Patent ↗An isolated RNA comprising an intron RNA that is released in a cell, thereby modulating the function of a target gene. Also disclosed are a composition comprising a chemokine and an isolated RNA of the invention or a DNA template for the isolated RNA, a composition comprising one or more agents that induce RNA-mediated modulation of the functions of two or more target genes in a cell, and methods of using these compositions for modulating the functions of genes in a cell.
1. An isolated RNA comprising:
an artificial intron comprising a splice donor site, a splice acceptor site, a branch site, a poly-pyrimidine tract and at least one aberrant RNA selected from the group consisting of a short-temporary RNA, small-interfering RNA, short-hairpin RNA, long deoxyribonucleotide-containing RNA and ribozyme RNA,
wherein said aberrant RNA is an RNA capable of silencing a target gene when released in a cell.
2. The isolated RNA of claim 1 , wherein the cell is a mammalian cell.
3. The isolated RNA of claim 1 , wherein the splice donor site contains 5′-AGGUAAGU-3′.
4. The isolated RNA of claim 1 , wherein the splice acceptor site contains 5′-CCACAGC-3′.
5. The isolated RNA of claim 1 , wherein the branch site contains 5′-UACUAAC-3.
6. The isolated RNA of claim 1 , wherein the cell is a eukaryotic cell.
7. An isolated RNA comprising:
an artificial intron RNA comprising at least one aberrant RNA selected from the group consisting of a short-temporary RNA, small-interfering RNA, short-hairpin RNA, long deoxyribonucleotide-containing RNA (D-RNA) and ribozyme RNA, said aberrant RNA being capable of silencing a target gene when released in a eukaryotic cell and wherein the artificial intron RNA further comprises a splice donor site that includes 5′-AGGUAAGU-3′, a splice acceptor site that includes 5′-CCACAGC-3′, a branch site that includes 5′-UACUAAC-3′, a poly-pyrimidine tract that includes 5′-UUCUUUUUUUC-3′ (SEQ ID NO:2), or a combination thereof.
8. A cultivated cell comprising the isolated RNA of claim 1 .
9. A composition comprising the isolated RNA of claim 1 .
10. The isolated RNA of claim 1 , wherein the artificial intron contains a sequence that targets an exon of the target gene, wherein the target gene is integrin β1.
11. The isolated RNA of claim 6 , wherein the artificial intron contains a sequence that targets an exon of the target gene, wherein the target gene is integrin β1.
12. The isolated RNA of claim 7 , wherein the artificial intron contains a sequence that targets an exon of the target gene, wherein the target gene is integrin β1.