Cell separation compositions and methods
View Patent ↗The invention provides compositions and methods for cell separation. These reagents and techniques specifically agglutinate cells via surface antigen recognition and can be used to recover even rare cell types in high yield.
1. A composition comprising:
a) dextran;
b) anti-glycophorin A antibody;
c) anti-CD15 antibody;
d) anti-CD9 antibody;
e) anti-CD94 antibody; and
f) anti-CD161 antibody.
2. The composition of claim 1 , said composition further comprising anti-CD72 antibody.
3. The composition of claim 1 , said composition further comprising anti-CD4 antibody, anti-CD16 antibody, and anti-CD19 antibody.
4. The composition of claim 1 , said composition further comprising anti-CD8 antibody, anti-CD16 antibody, and anti-CD19 antibody.
5. The composition of claim 1 , said composition further comprising anti-CD2 antibody.
6. The composition of claim 1 , further comprising heparin.
7. The composition of claim 1 , further comprising divalent cations.
8. The composition of claim 7 , wherein said divalent cations are Ca +2 .
9. The composition of claim 7 , wherein said divalent cations are Mg +2 .
10. The composition of claim 1 , further comprising phosphate buffered saline.
11. The composition of claim 1 , wherein the pH of said composition is between 6.8 to 7.8.
12. The composition of claim 1 , wherein the pH of said composition is between 7.2 to 7.4.
13. The composition of claim 1 , wherein said anti-glycophorin A antibody is monoclonal.
14. The composition of claim 1 , wherein said anti-glycophorin A antibody is an IgM antibody, or an IgG antibody.
15. The composition of claim 1 , wherein said anti-glycophorin A antibody is an anti-human glycophorin A antibody.
16. The composition of claim 1 , wherein the concentration of said anti-glycophorin A antibody is about 0.1 mg/L to about 15 mg/L.
17. The composition of claim 1 , wherein said anti-CD15 antibody is a monoclonal antibody.
18. The composition of claim 1 , wherein said anti-CD15 antibody is an IgM antibody or an IgG antibody.
19. The composition of claim 1 , wherein said anti-CD15 antibody is an anti-human CD15 antibody.
20. The composition of claim 1 , wherein the concentration of said anti-CD15 antibody is about 0.1 mg/L to about 15 mg/L.
21. The composition of claim 1 , wherein said anti-CD9 antibody is a monoclonal antibody.
22. The composition of claim 1 , wherein said anti-CD9 antibody is an IgM antibody, or an IgG antibody.
23. The composition of claim 1 , wherein said anti-CD9 antibody is an anti-human CD9 antibody.
24. The composition of claim 1 , wherein the concentration of said anti-CD9 antibody is at about 0.1 mg/L to about 15 mg/L.
25. The composition of claim 1 , wherein the concentration of said anti-CD94 antibody is 0.01 mg/L to 10 mg/L.
26. The composition of claim 1 , wherein the concentration of said anti-CD161 antibody is 0.01 mg/L to 10 mg/L.
27. The composition of claim 1 , wherein said anti-CD94 antibody is an IgM or IgG antibody.
28. The composition of claim 1 , wherein said anti-CD161 antibody is an IgM or IgG antibody.
29. The composition of claim 1 , wherein said anti-CD94 antibody is an anti-human CD94 antibody.
30. The composition of claim 1 , wherein said anti-CD161 antibody is an anti-human CD161 antibody.
31. A composition comprising:
a) dextran;
b) anti-glycophorin A antibody;
c) anti-CD15 antibody;
d) anti-CD9 antibody;
e) anti-CD4 antibody or anti-CD8 antibody;
f) anti-CD16 antibody;
g) anti-CD19 antibody;
h) anti-CD94 antibody;
i) anti-CD161 antibody;
j) heparin; and
k) divalent cations.
32. The composition of claim 31 , wherein said composition contains anti-CD4 antibody.
33. The composition of claim 31 , wherein said composition contains anti-CD8 antibody.
34. The composition of claim 31 , wherein at least one of said antibodies is biotinylated.
35. The composition of claim 34 , wherein said anti-CD16 antibody, said anti-CD19 antibody, said anti-CD94 antibody, and said anti-CD161 antibody are biotinylated.
36. A kit comprising a blood collection vessel and the cell separation composition of claim 31 .
37. The kit of claim 36 , wherein said blood collection vessel is a blood bag.
38. The kit of claim 36 , wherein said blood collection vessel is a vacuum tube.
39. A method for separating cells, said method comprising:
a) contacting a blood cell-containing sample with a composition, said composition comprising:
i) dextran;
ii) anti-glycophorin A antibody;
iii) anti-CD15 antibody;
iv) anti-CD9 antibody;
v) anti-CD94 antibody; and
vi) anti-CD161 antibody.
b) allowing said sample to partition into an agglutinate and a supernatant phase; and
c) recovering cells from either or both the agglutinate or supernatant phase.
40. The method of claim 39 , wherein said composition further comprises anti-CD4 antibody, anti-CD16 antibody, and anti-CD19 antibody.
41. The method of claim 39 , wherein said composition further comprises anti-CD8 antibody, anti-CD16 antibody, and anti-CD19 antibody.
42. The method of claim 39 , wherein said sample is derived from a human.
43. The method of claim 42 , wherein said sample is a peripheral blood sample.
44. The method of claim 42 , wherein said sample is an umbilical cord sample.
45. The method of claim 42 , wherein said sample is a bone marrow sample.
46. The method of claim 42 , wherein said cells are metastatic tumor cells.
47. The method of claim 42 , wherein said cells are recovered from said supernatant phase.
48. The method of claim 47 , wherein said cells are stem cells.
49. The method of claim 39 , wherein said anti-CD94 antibody and said anti-CD161 antibody are biotinylated.
50. The method of claim 49 , wherein recovery of said cells comprises contacting said supernatant phase with a composition comprising avidin-coated particles, separating said supernatant phase and said composition comprising avidin-coated particles, and recovering cells from said supernatant phase.