IP Library › Granted Patent US 7,575,929
Granted Patent B2
US 7,575,929 · App. 10/679,699 · Granted Aug 18, 2009

Diagnosis of multiple sclerosis with diketopiperazines

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Quick Facts
Patent No.
US 7,575,929
App. No.
10/679,699
Granted
Aug 18, 2009
Kind
B2
Abstract

The present invention relates to the diagnosis and monitoring of diseases and conditions by quantifying markers, including degradation products of disease-associated proteins, such as diketopiperazines composed of the two N-terminal amino acids or the two C-terminal amino acids of such proteins. The methods are useful for diagnosing or monitoring various diseases, including multiple sclerosis, Alzheimer's disease and ischemia. The invention further provides binding partners specific for the markers and compositions and kits for conducting the methods of the invention.

Claims (12)

1. A method of diagnosing multiple sclerosis (MS) comprising the steps of:

(a) obtaining a biological sample from a patient, wherein the biological sample is serum, plasma or blood;

(b) measuring the amount of one or more MS diagnostic compounds in the biological sample, the MS diagnostic compound(s) being:

(i) aspartic acid-alanine diketopiperazine (DA-DKP);

(ii) N-acetyl-alanine-serine diketopiperazine (NAS-DKP); or

(iii) both of the foregoing;

wherein

an elevated amount of DA-DKP, NAS-DKP or both DA-DKP and NAS-DKP in the biological sample is indicative of active MS.

2. The method of claims 1 wherein the biological sample is serum or plasma.

3. The method of claim 2 , wherein the biological sample is plasma.

4. The method of claim 2 , wherein the biological sample is serum.

5. The method of any one of claims 1 and 2 - 4 wherein step (b) is conducted by mass spectrometry, immunoassay or both.

Assignments (8)
CERTIFICATES OF CONVERSION AND INCORPORATION Recorded Aug 20, 2015
From: ROSEWIND CORPORATION
To: AYTU BIOSCIENCE, INC.
Reel/Frame 036400/0479 →
MERGER Recorded Aug 20, 2015
From: LUOXIS DIAGNOSTICS, INC.
To: ROSEWIND CORPORATION
Reel/Frame 036380/0283 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 11, 2013
From: DMI ACQUISITION CORP.
To: LUOXIS DIAGNOSTICS, INC.
Reel/Frame 030198/0883 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 4, 2013
From: DMI BIOSCIENCES, INC.
To: DMI ACQUISITION CORP.
Reel/Frame 029565/0273 →
SECOND LIEN INTELLECTUAL PROPERTY SECURITY AGREEMENT Recorded Jun 27, 2007
From: IM US HOLDINGS, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL INNOVATIONS, INC., A DELAWARE CORPORATION; ADVANTAGE DIAGNOSTICS CORPORATION, A DELAWARE CORPORATION; APPLIED BIOTECH, INC., A CALIFORNIA CORPORATION; BINAX, INC., A DELAWARE CORPORATION; FIRST CHECK DIAGNOSTICS CORP., A DELAWARE CORPORATION; FOREFRONT DIAGNOSTICS, INC., A CALIFORNIA CORPORATION; INCA ACQUISITION, INC., A DELAWARE CORPORATION; INNOVATIVE MOBILITY, LLC, A FLORIDA LIMITED LIABILITY COMPANY; INSTANT TECHNOLOGIES, INC., A VIRGINIA CORPORATION; INVERNESS MEDICAL - BIOSTAR, INC., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP. II, A DELAWARE CORPORATION; INNOVATIONS RESEARCH, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL, LLC, A DELAWARE LIMITED LIABILITY COMPANY; ISCHEMIA TECHNOLOGIES, INC., A DELAWARE CORPORATION; IVC INDUSTRIES, INC., A DELAWARE CORPORATION; INNOVACON, INC., A DELAWARE CORPORATION; OSTEX INTERNATIONAL, INC., A WASHINGTON CORPORATION; QUALITY ASSURED SERVICES, INC., A FLORIDA CORPORATION; SELFCARE TECHNOLOGY, INC., A DELAWARE CORPORATION; SPDH, INC., A DELAWARE CORPORATION; UNIPATH ONLINE, INC., A MASSACHUSETTS CORPORATION; WAMPOLE LABORATORIES, LLC, A DELAWARE LIMITED LIABILITY COMPANY
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT
Reel/Frame 019477/0808 →
FIRST LIEN INTELLECTUAL PROPERTY SECURITY AGREEMENT Recorded Jun 26, 2007
From: IM US HOLDINGS, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL INNOVATIONS, INC., A DELAWARE CORPORATION; ADVANTAGE DIAGNOSTICS CORPORATION, A DELAWARE CORPORATION; APPLIED BIOTECH, INC., A CALIFORNIA CORPORATION; BINAX, INC., A DELAWARE CORPORATION; FIRST CHECK DIAGNOSTICS CORP., A DELAWARE CORPORATION; FOREFRONT DIAGNOSTICS, INC., A CALIFORNIA CORPORATION; INCA ACQUISITION, INC., A DELAWARE CORPORATION; INNOVATIVE MOBILITY, LLC, A FLORIDA LIMITED LIABILITY COMPANY; INSTANT TECHNOLOGIES, INC., A VIRGINIA CORPORATION; INVERNESS MEDICAL - BIOSTAR, INC., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP. II, A DELAWARE CORPORATION; INNOVATIONS RESEARCH, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL, LLC, A DELAWARE LIMITED LIABILITY COMPANY; ISCHEMIA TECHNOLOGIES, INC., A DELAWARE CORPORATION; IVC INDUSTRIES, INC., A DELAWARE CORPORATION; INNOVACON, INC., A DELAWARE CORPORATION; OSTEX INTERNATIONAL, INC., A WASHINGTON CORPORATION; SELFCARE TECHNOLOGY, INC., A DELAWARE CORPORATION; SPDH, INC., A DELAWARE CORPORATION; UNIPATH ONLINE, INC., A MASSACHUSETTS CORPORATION; QUALITY ASSURED SERVICES, INC., A FLORIDA CORPORATION
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT
Reel/Frame 019477/0268 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 28, 2004
From: BAR-OR, DAVID
To: DMI BIOSCIENCES, INC.
Reel/Frame 015511/0148 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 14, 2004
From: BAR-OR, DAVID; BAR-OR, RAPHAEL
To: DMI BIOSCIENCES, INC.
Reel/Frame 015456/0696 →