Process for delivery of polynucleotides to the prostate
Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.
1. A process for delivering DNA to a prostate cell in a mammal comprising:
a) inserting a delivery device into a lumen of an afferent or efferent vessel of the prostate;
b) injecting a solution containing the DNA into the lumen of the vessel;
c) increasing permeability of the vessel to the DNA;
d) delivering the DNA to the prostate cell outside of the vessel; and,
e) expressing the DNA.
2. The process of claim 1 wherein fluid flow out of the prostate tissue is impeded by occlusion of at least one vessel.
3. The process of claim 1 wherein the vessel consists of a blood vessel.
4. The process of claim 3 wherein the blood vessel consists of a vein.
5. The process of claim 3 wherein the blood vessel consists of an artery.
6. The process of claim 4 wherein the vein is selected from the list consisting of: prostatic venous plexus, prostatic vein, tributaries of the deep dorsal vein of the penis, dorsal vein of the penis, internal iliac vein, and vesical venous plexus, and their tributaries.
7. The process of claim 5 wherein the artery is selected from the list consisting of: inferior vesical artery, internal iliac artery, prostatic artery, internal pudic artery, hemorrhoidal artery, and rectal artery, and their tributaries.
8. The process of claim 1 wherein expression of the DNA induces an immune response.
9. The process of claim 1 wherein the DNA is associated with a transfection agent.
10. The process of claim 1 wherein the solution contains a compound that increases vascular permeability.
11. The process of claim 1 wherein expression of the DNA alters the endogenous properties of the cell.
12. The process of claim 1 wherein the mammal has benign prostatic hyperplasia.
13. The process of claim 1 wherein the mammal has prostate cancer.