Anti-APO-B-48 monoclonal antibody, hybridoma producing the same, and method for measuring APO-B-48 using the same
View Patent ↗A novel monoclonal antibody which specifically binds to apo-B-48 is disclosed. The monoclonal antibody specifically binds to apo-B-48 but does not bind to apo-B-100.
1. A method for measuring apo-B-48 in a test sample, comprising:
a) incubating the test sample with a monoclonal antibody that specifically bind to apo-B-48 and which does not bind to apo-B-100;
b) allowing a complex between apo-B-48 and the antibody to form;
c) adding a labeled second antibody capable of binding to apo-B-48;
d) detecting resultant labeled complexes; and
e) correlating the detected complex with the presence of apo-B-48 in the test sample;
wherein apo-B-48 in said test sample is subjected to a treatment with one or more nonionic surfactants by which the corresponding epitope of said monoclonal antibody is exposed before or simultaneously with an antigen-antibody reaction.
2. The method according to claim 1 , wherein said monoclonal antibody is immobilized on a solid carrier, and apo-B-48 immobilized on said carrier through said monoclonal antibody is measured after said antigen-antibody reaction.
3. The method according to claim 2 , which is carried out by ELISA.