Dry powder cells and cell culture reagents and methods of production thereof
View Patent ↗Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.
1. A method of producing an agglomerated eukaryotic medium powder, said method comprising agglomerating a eukaryotic dry powder medium with a solvent, wherein the solvent comprises at least one lipid, wherein the agglomerated eukaryotic medium powder exhibits reduced dusting and a larger particle size than does the eukaryotic dry powder medium.
2. The method of claim 1 , further comprising packaging said agglomerated powder.
3. The method of claim 1 , further comprising sterilizing said agglomerated powder.
4. The method of claim 3 , wherein said sterilization is performed after packaging said agglomerated powder.
5. The method of claim 3 , wherein said sterilization is accomplished by irradiation of said agglomerated powder with gamma rays until said agglomerated powder is rendered substantially sterile.
6. The method of claim 1 , wherein when said agglomerated eukaryotic medium powder is reconstituted with water, a reconstituted eukaryotic medium at a desired pH for culturing a eukaryotic cell is produced.
7. The method of claim 1 , wherein said agglomerated eukaryotic medium powder comprises epidermal growth factor (EGF), acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2) or nerve growth factor (NGF).
8. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing an animal cell.
9. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing a mammalian cell.
10. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing a human cell.
11. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing a hybridoma cell.
12. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing a cell selected from the group consisting of an insect cell, a nematode cell, a fungal cell, a plant cell and a yeast cell.
13. The method of claim 1 , wherein the agglomerated eukaryotic medium powder is for culturing a cell selected from the group consisting of an embryonic cell, a Drosophila cell, a Spodoptera cell, a Trichoplusa cell, a Caenorhabditis elegans cell, a Chinese hamster ovary (CHO) cell, a COS cell, a VERO cell, a baby hamster kidney (BHK) cell, an alveolar epithelial type 1 (AE-1) cell, a SP2/0 cell and a L5.1 cell.
14. The method of claim 1 , wherein the at least one lipid is a phospholipid.
15. The method of claim 1 , wherein the at least one lipid is a sphingolipid.
16. The method of claim 1 , wherein the at least one lipid is a fatty acid.
17. The method of claim 1 , wherein the at least one lipid is a cholesterol.
18. The method of claim 1 , comprising
(a) placing the eukaryotic dry powder medium into a fluid bed apparatus;
(b) introducing the solvent into the eukaryotic dry powder medium under conditions whereby the eukaryotic dry powder medium is moistened to obtain a moistened eukaryotic dry powder medium; and
(c) drying the moistened eukaryotic dry powder medium, thereby producing an agglomerated eukaryotic medium powder.