Methods for drug discovery, disease treatment, and diagnosis using metabolomics
View Patent ↗The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.
1. A method for metabolomically identifying small molecules indicative of amyotrophic lateral sclerosis, comprising:
obtaining a small molecule profile from a subject suffering from amyotrophic lateral sclerosis; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of amyotrophic lateral sclerosis, wherein said small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
2. The method of claim 1 , wherein said subject is a human.
3. The method of claim 1 , wherein said small molecule profile is obtained from said subject's tissue.
4. The method of claim 1 , wherein said small molecule profile is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, gas chromatography (GC), radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), and Light Scattering analysis (LS).
5. A method for metabolomically identifying small molecules indicative of Parkinson's disease, comprising:
obtaining a small molecule profile from a subject suffering from Parkinson's disease; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of Parkinson's disease, wherein said small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
6. A method for metabolomically identifying small molecules indicative of schizophrenia, comprising:
obtaining a small molecule profile from a subject suffering from schizophrenia; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of schizophrenia, wherein said small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
7. A method for metabolomically identifying small molecules indicative of Parkinson's disease, comprising:
obtaining a small molecule profile from a subject suffering from Parkinson's disease; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of Parkinson's disease, wherein small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
8. A method for metabolomically identifying small molecules indicative of depression, comprising:
obtaining a small molecule profile from a subject suffering from depression; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of depression, wherein said small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
9. A method for metabolomically identifying small molecules indicative of schizophrenia, comprising:
obtaining a small molecule profile from a subject suffering from schizophrenia; and
comparing the small molecule profile from the subject to a standard small molecule profile, thereby identifying small molecules indicative of schizophrenia, wherein small molecule profile is obtained using one or more techniques which detect 50% or more of the small molecules in said sample.
10. The method of claim 4 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
11. The method of claim 5 , wherein said subject is a human.
12. The method of claim 5 , wherein said small molecule profiles is obtained from said subject's tissue.
13. The method of claim 5 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
14. The method of claim 5 , wherein said small molecule profiles is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), gas chromatography (GC) and Light Scattering analysis (LS).
15. The method of claim 6 , wherein said subject is a human.
16. The method of claim 6 , wherein said small molecule profiles is obtained from said subject's tissue.
17. The method of claim 6 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
18. The method of claim 6 , wherein said small molecule profiles is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), gas chromatography (GC) and Light Scattering analysis (LS).
19. The method of claim 7 , wherein said subject is a human.
20. The method of claim 7 , wherein said small molecule profiles is obtained from said subject's tissue.
21. The method of claim 7 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
22. The method of claim 7 , wherein said small molecule profiles is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), gas chromatography (GC) and Light Scattering analysis (LS).
23. The method of claim 8 , wherein said subject is a human.
24. The method of claim 8 , wherein said small molecule profiles is obtained from said subject's tissue.
25. The method of claim 8 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
26. The method of claim 8 , wherein said small molecule profiless is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), gas chromatography (GC) and Light Scattering analysis (LS).
27. The method of claim 9 , wherein said subject is a human.
28. The method of claim 9 , wherein said small molecule profiles is obtained from said subject's tissue.
29. The method of claim 9 , wherein said small molecule profiles is obtained from said subject's cells, cellular organelles, interstitial fluid, or saliva.
30. The method of claim 9 , wherein said small molecule profiles is obtained using one or more of the following: HPLC, TLC, electrochemical analysis, mass spectroscopy, refractive index spectroscopy (RI), Ultra-Violet spectroscopy (UV), fluorescent analysis, radiochemical analysis, Near-InfraRed spectroscopy (Near-IR), Nuclear Magnetic Resonance spectroscopy (NMR), gas chromatography (CC) and Light Scattering analysis (LS).
31. The method of claim 4 , wherein said small molecule profile is obtained from said subject's biological fluids.
32. The method of claim 1 , wherein said small molecule profile is obtained using mass spectroscopy.
33. The method of claim 1 , wherein said small molecule profile is obtained using gas chromatography (GC).
34. The method of claim 1 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
35. The method of claim 1 , wherein said small molecule profile is obtained using HPLC.
36. The method of claim 4 , wherein said small molecule profile is obtained from said subject's spinal fluid.
37. The method of claim 4 , wherein said small molecule profiles are obtained from said subject's serum.
38. The method of claim 4 , wherein said small molecule profile is obtained from said subject's urine.
39. The method of claim 5 , wherein said small molecule profile is obtained from said subject's biological fluids.
40. The method of claim 14 , wherein said small molecule profile is obtained using mass spectroscopy.
41. The method of claim 14 , wherein said small molecule profile is obtained using gas chromatography (GC).
42. The method of claim 14 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
43. The method of claim 14 , wherein said small molecule profile is obtained using HPLC.
44. The method of claim 5 , wherein said small molecule profiles are obtained from said subject's serum.
45. The method of claim 5 , wherein said small molecule profile is obtained from said subject's urine.
46. The method of claim 5 , wherein said small molecule profile is obtained from said subject's spinal fluid.
47. The method of claim 6 , wherein said small molecule profile is obtained from said subject's biological fluids.
48. The method of claim 18 , wherein said small molecule profile is obtained using mass spectroscopy.
49. The method of claim 18 , wherein said small molecule profile is obtained using gas chromatography (GC).
50. The method of claim 18 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
51. The method of claim 18 , wherein said small molecule profile is obtained using HPLC.
52. The method of claim 6 , wherein said small molecule profile is obtained from said subject's spinal fluid.
53. The method of claim 6 , wherein said small molecule profiles are obtained from said subject's serum.
54. The method of claim 6 , wherein said small molecule profile is obtained from said subject's urine.
55. The method of claim 7 , wherein said small molecule profile is obtained from said subject's biological fluids.
56. The method of claim 22 , wherein said small molecule profile is obtained using mass spectroscopy.
57. The method of claim 22 , wherein said small molecule profile is obtained using gas chromatography (GC).
58. The method of claim 22 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
59. The method of claim 22 , wherein said small molecule profile is obtained using HPLC.
60. The method of claim 7 , wherein said small molecule profile is obtained from said subject's spinal fluid.
61. The method of claim 7 , wherein said small molecule profiles are obtained from said subject's serum.
62. The method of claim 7 , wherein said small molecule profile is obtained from said subject's urine.
63. The method of claim 8 , wherein said small molecule profile is obtained from said subject's biological fluids.
64. The method of claim 26 , wherein said small molecule profile is obtained using mass spectroscopy.
65. The method of claim 26 , wherein said small molecule profile is obtained using gas chromatography (GC).
66. The method of claim 26 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
67. The method of claim 26 , wherein said small molecule profile is obtained using HPLC.
68. The method of claim 8 , wherein said small molecule profile is obtained from said subject's spinal fluid.
69. The method of claim 8 , wherein said small molecule profiles are obtained from said subject's serum.
70. The method of claim 8 , wherein said small molecule profile is obtained from said subject's urine.
71. The method of claim 9 , wherein said small molecule profile is obtained from said subject's biological fluids.
72. The method of claim 30 , wherein said small molecule profile is obtained using mass spectroscopy.
73. The method of claim 30 , wherein said small molecule profile is obtained using gas chromatography (GC).
74. The method of claim 30 , wherein said small molecule profile is obtained using Nuclear Magnetic Resonance spectroscopy (NMR).
75. The method of claim 30 , wherein said small molecule profile is obtained using 1 {PLC.
76. The method of claim 9 , wherein said small molecule profile is obtained from said subject's spinal fluid.
77. The method of claim 9 , wherein said small molecule profiles are obtained from said subject's serum.
78. The method of claim 9 , wherein said small molecule profile is obtained from said subject's urine.