Meningococcal antigens
View Patent ↗The invention provides proteins from Neisseria meningitidis (strains A & B), including amino acid sequences, the corresponding nucleotide sequences, expression data, and serological data. The proteins are useful antigens for vaccines, immunogenic compositions, and/or diagnostics.
1. An isolated nucleic acid molecule which encodes a protein comprising an amino acid sequence of SEQ ID NO:4.
2. A nucleic acid molecule according to claim 1 , comprising a nucleotide sequence of SEQ ID NO:3.
3. An isolated nucleic acid molecule encoding an immunogenic polypeptide having 90% or greater sequence identity to an amino acid sequence of SEQ ID NO:4, wherein the immunogenic polypeptide can raise antibodies which specifically bind to SEQ ID NO:4.
4. An isolated nucleic acid molecule comprising a fragment of 25 or more nucleotides of a nucleotide sequence of SEQ ID NO:3, wherein the fragment is capable of detecting N. meningitidis nucleic acids through hybridization under high stringency conditions comprising a wash in 0.1×SSC, 0.5% SDS solution at 65° C.
5. An isolated nucleic acid molecule encoding an immunogenic polypeptide, wherein the isolated nucleic acid molecule can hybridize to a nucleic acid molecule which encodes a protein comprising an amino acid sequence of SEQ ID NO:4 under high stringency conditions comprising a wash in 0.1×SSC, 0.5% SDS solution at 65° C., wherein the isolated nucleic acid molecule is capable of detecting N. meningitidis nucleic acids through hybridization.
6. An isolated nucleic acid molecule that encodes a protein comprising an immunogenic fragment of at least 20 consecutive amino acids of an amino acid sequence of SEQ ID NO:4, wherein the immunogenic fragment can raise antibodies which specifically bind to SEQ ID NO:4.
7. The isolated nucleic acid molecule of claim 3 wherein the immunogenic polypeptide has 99% or greater sequence identity to an amino acid sequence of SEQ ID NO:4.
8. The isolated nucleic acid molecule of claim 3 wherein the immunogenic polypeptide has 95% or greater sequence identity to an amino acid sequence of SEQ ID NO:4.