PTPN11 (SHP-2) mutations and cancer
View Patent ↗Diagnostic and therapeutic applications for certain types of cancer and precancerous conditions, including those deriving from hematologic cells, are described. Of particular interest are those cancers and precancerous conditions associated with increased signaling in the RAS-MAP kinase pathway. The diagnostic and therapeutic applications described herein are based on certain mutations in the protein tyrosine phosphatase gene PTPN11 and its expression product, PTPN11, promoting a gain-of-function in PTPN11 activity. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to PTPN11 and PTPN11 variants, and cells expressing such variants.
1. A method for characterizing a hematologic disorder in a human subject comprising detecting in a sample from the subject the presence of a mutation in the subject's protein tyrosine phosphatase 11 (PTPN11) gene encoding any of the following amino acid substitutions in PTPN11: Glu76Gly, Glu76Val, Glu76Ala, Glu76Gln and Glu76Lys, wherein Glu76Gly is indicative of juvenile myelomonocytic leukemia (JMML) or acute lymphoblastic leukemia (ALL), Glu76Val is indicative of JMML, Glu76Ala is indicative of JMML or myelodysplastic syndrome (MDS), Glu76Gln is indicative of ALL, and Glu76Lys is indicative of JMML, ALL, or acute myeloid leukemia (AML).
2. The method of claim 1 , wherein said detecting comprises a method selected from the group consisting of antibody binding, oligonucleotide sequencing, Denaturing Gradient Gel Electrophoresis, Single Strand Conformation Polymorphism, HOT cleavage, reverse-transcription POR, microarray analysis, immunoassay, POR, and restriction fragmentation.