Rapid growing microorganisms for biotechnology applications
View Patent ↗The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.
1. An isolated, competent E. coli that exhibits visible colonies 7.8 to 8.5 hours after transformation and incubation on LB agar at 37° C., wherein said E. coli is suitable for alpha-complementation and selection by blue/white screening.
2. The competent E. coli according to claim 1 , wherein said colonies are 1 mm in size.
3. The competent E. coli according to claim 1 , wherein said E. coli is a K12 derivative.
4. The competent E. coli according to claim 1 , wherein said E. coli is lacZ − .
5. The competent E. coli according to claim 4 , wherein said E. coli is lacZ − .
6. The competent E. coli according to claim 1 , wherein said E. coli is recA − .
7. The competent E. coli according to claim 1 , wherein said E. coli is restriction negative.
8. The competent E. coli according to claim 1 , wherein said E. coli is modification negative.
9. The competent E. coli according to claim 1 , wherein said E. coli is restriction negative and modification negative.
10. The competent E. coli according to claim 1 , wherein said E. coli is methylase deficient.
11. The competent E. coli according to claim 10 , wherein said E. coli is mcrA − .
12. The competent E. coli according to claim 10 , wherein said E. coli is mcrB − .
13. The competent E. coli according to claim 1 , wherein said E. coli is endonuclease deficient.
14. The competent E. coli according to claim 13 , wherein said E. coli is endA1.
15. The competent E. coli according to claim 3 , wherein said E. coli is lacZ − , restriction negative, methylase deficient, and endonuclease deficient.
16. The competent E. coli according to claim 15 , wherein said E. coli is lacZ − , mcrB − , and endA1.