Isolation and confirmation of analytes from test devices
View Patent ↗Methods, articles, and kits for obtaining elevated concentrations of a substance from a sample that has become bound, for example by providing a positive test result in an immunoassay or other binding assay. The concentrated substance is optionally subjected to further processing or analysis, for example to confirm the identity of the substance, such as a pathogenic organism in a food sample.
1. A method for detecting, isolating or purifying a bacteria comprising:
placing a sample in contact with a device, wherein at least a portion of the device comprises a plurality of zones, wherein at least one zone is a detection zone separable from said plurality of zones and the remainder of the device; wherein the detection zone comprises an immobilized binding partner for a bacteria and wherein binding between the immobilized binding partner and a suspected bacteria causes formation of an optically detectable signal and detection of the signal indicates the presence of a suspected bacteria in the sample;
separating at least part of the detection zone containing the bound bacteria and immobilized binding partner from said plurality of zones and the remainder of the device; and
analyzing the separated detection zone containing the bound bacteria and immobilized binding partner without detaching the bound bacteria from the immobilized binding partner to provide information regarding the suspected bacteria.
2. The method of claim 1 , wherein the device is a lateral flow device.
3. The method of claim 1 , wherein the information identifies the suspected bacteria.
4. The method of claim 1 , wherein the information describes one or more characteristics of the suspected bacteria.
5. The method of claim 1 , further comprising:
placing the separated detection zone containing the bound bacteria and immobilized binding partner in conditions effective to cause the quantity of the suspected bacteria to increase.
6. The method of claim 1 , wherein analyzing the separated detection zone containing the bound bacteria and immobilized binding partner comprises placing the separated detection zone containing the bound bacteria and immobilized binding partner on or in a selective growth medium in which the bacteria will proliferate if present.
7. The method of claim 1 , wherein the method further comprises storing the device without further processing for up to five days after placing the sample in contact with the device and before separating the detection zone containing the bound bacteria and immobilized binding partner from the plurality of zones and the remainder of the device.
8. A kit for performing the method of claim 1 , comprising a device; wherein at least a portion of the device comprises a plurality of zones, wherein at least one zone is a detection zone; wherein the, detection zone comprises an immobilized binding partner for a bacteria, wherein the detection zone is separable from the plurality of zones and the remainder of the device and, wherein the separated detection zone is analyzed to provide information regarding the bound bacteria.
9. The method of claim 1 , wherein the bacteria further comprises a food or environmental contaminant.
10. The method of claim 1 , wherein the bacteria is a pathogen.
11. The method of claim 1 , wherein analyzing the separated detection zone containing the bound bacteria and immobilized binding partner to provide information regarding the suspected bacteria comprises analyzing the separated detection zone containing the bound bacteria and immobilized binding partner using a strip test binding assay, an agglutination assay, a DNA polymerase chain reaction test, a motility test, a toxicology test, and serotyping.
12. The method of claim 1 , wherein analyzing the separated detection zone containing the bound bacteria and immobilized binding partner to provide information regarding the suspected bacteria comprises analyzing the separated detection zone containing the bound bacteria and immobilized binding partner using a DNA polymerase chain reaction test.
13. The method of claim 1 , wherein analyzing the separated detection zone containing the bound bacteria and immobilized binding partner to provide information regarding the suspected bacteria comprises analyzing the separated detection zone containing the bound bacteria and immobilized binding partner using selective media or selective agar plating.
14. The method of claim 1 , wherein the bacteria is Escherichia coli, Salmonella or Listeria.
15. The method of claim 14 , wherein the bacteria is Escherichia coli O157.
16. The method of claim 1 , wherein the optically detectable signal is a light signal.
17. A device for detecting bacteria, wherein at least a portion of the device comprises a plurality of zones, wherein at least one zone is a detection zone separable from said plurality of zones and the remainder of the device; wherein the detection zone comprises an immobilized binding partner for a bacteria and wherein binding between the immobilized binding partner and a suspected bacteria causes formation of an optically detectable signal in the detection zone, and wherein the device comprises structural features that facilitate separation of the detection zone containing the bound bacteria and the immobilized binding partner or a part of the detection zone containing the bound bacteria and the immobilized binding partner from the plurality of zones and the remainder of the device, wherein said separated detection zone or part thereof can be analyzed to provide information regarding the bound bacteria.
18. The device of claim 17 , wherein the device is a lateral flow device.
19. The device of claim 17 , wherein the optically detectable signal in the detection zone is a light signal.
20. A method for detecting, isolating or concentrating a bacteria by placing a sample containing the bacteria under conditions effective to cause proliferation, replication or reproduction of the bacteria comprising:
placing a sample in contact with a device, wherein at least a portion of the device comprises a plurality of zones, wherein at least one zone is a detection zone capable of being separated from said plurality of zones and the remainder of the device; wherein the detection zone comprises an immobilized binding partner for a bacteria and wherein binding between the immobilized binding partner and a suspected bacteria causes formation of an optically detectable signal and detection of the signal indicates the presence of a suspected bacteria in the sample;
separating at least part of the detection zone containing the bound bacteria and immobilized binding partner from said plurality of zones and the remainder of the device; and
subjecting the separated detection zone to conditions effective to cause proliferation, replication or reproduction of the bacteria.
21. The method of claim 20 , wherein the device is a lateral flow device.
22. The method of claim 20 , wherein the information identifies the suspected bacteria.
23. The method of claim 20 , wherein the information describes one or more characteristics of the suspected bacteria.