Element for protein microarrays
View Patent ↗An element for the attachment of protein arrays, the element comprising a surface to which are attached a plurality of piperazine functional groups wherein the piperazine functional groups are represented by Formula I: where R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , are hydrogen, alkyl, alkenyl, alkynyl, alkylhalo, cycloalkyl, cycloalkenyl, alkylthio, alkoxy, with the proviso that at least one of R 1 to R 10 be a non-labile chemical unit that attaches the piperazine functional group to the surface of the element.
1. An element for the attachment of protein arrays, the element comprising a surface to which are attached
a plurality of piperazine functional groups;
a polymer;
a crosslinking compound A-L-B;
wherein A is a functional group capable of interacting with a piperazine functional group of the invention; L is a linking group capable of interacting with A and with B; and B is a specific functionality that provides one or more reactive units capable of interacting with a protein capture agent, and wherein the piperazine functional groups are represented by Formula I:
where
R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , are hydrogen, alkyl, alkenyl, alkynyl, alkylhalo, cycloalkyl, cycloalkenyl, alkylthio, alkoxy, with the proviso that at least one of the groups R 1 to R 10 be a linkage group (L′).
2. The element in claim 1 wherein at least one of the functional groups A or B in A-L-B is aldehyde, epoxy, hydrazide, vinylsulfone, succinimidyl ester, carbodiimide, maleimide, dithio, iodoacetyl, isocyanate, isothiocyanate, or aziridine.
3. The element in claim 2 wherein at least one of the functional groups in A-L-B is vinylsulfone.
4. The element of claim 1 wherein the linkage group L′ comprises any combination of non-labile covalently bonded chemical units sufficient to connect the piperazine functional group to the surface.
5. The element of claim 4 wherein the surface contains a polymer.
6. The element of claim 1 wherein the crosslinking compound A-L-B is bis(vinylsulfonyl)methane, bis(vinylsulfonyl)methyl ether, or bis(vinylsulfonylacetamido)ethane.
7. The element in claim 1 where the crosslinking compound A-L-B is bis(vinylsulfonyl)methane and the piperazine containing polymer comprising 1-(4-vinylbenzyl)piperazine or methacrylic acid piperazine amide monomers, or the corresponding inorganic acid or organic acid addition salts thereof.
8. The element of claim 1 wherein the crosslinking compound A-L-B is bis(vinylsulfonyl)methane and the piperazine polymer is defined by Formula II.
where 20, 30, and 50 represent the relative molar amounts of each monomeric unit.
9. The element of claim 7 wherein the surface contains gelatin.
10. The element of claim 8 wherein the surface contains gelatin.