Nephelometric detection unit with optical in-process control
View Patent ↗The present invention relates to the field of the use of automated measurement systems in analysis and in in-vitro diagnosis. In particular, the apparatus described enables automatic quality control and validation of characteristic process engineering parameters, in particular characteristic optical parameters, during the measurement of scattered light signals.
1. A method of obtaining a relatively consistent scattered light measurement, comprising:
moving an accommodation vessel through a light beam via a rotor system;
directing the light beam passing through the accommodation vessel toward a detection unit to produce a scattered component and a transmitted component of the light beam;
cyclically measuring, via a first detector, an intensity of the transmitted component of the light beam based on a position of the accommodation vessel relative to the light beam;
cyclically measuring, via second detector, an intensity of the scattered component of the light beam separately from the transmitted component based on the position of the accommodation vessel relative to the light beam;
adjusting an intensity of the light beam directed through the accommodation vessel based on the measured intensity of the transmitted component of the light beam; and
allowing the scattered component of the light beam to pass around a diaphragm upon which the transmitted component of the light beam impinges.
2. The method of claim 1 , wherein the intensity of the transmitted component of the light beam is measured by a detector mounted on the diaphragm.
3. The method of claim 1 , further comprising passing the transmitted component and the scattered component of the light beam through a first lens system.
4. The method of claim 1 , further comprising passing the scattered component of the light beam through a second lens system.
5. The method of claim 1 , further comprising separating the transmitted component of the light beam from the scattered component of the optical beam with a shaped diaphragm.
6. The method of claim 1 , wherein the diaphragm includes a region for mounting a detector.
7. The method of claim 1 , wherein the diaphragm includes a region for mounting a beam guidance or deflection unit.
8. The method of claim 1 , further comprising separating the transmitted component of the light beam from the scattered component of the light beam by a mirror placed in a path of the light beam, a beam guidance or deflection unit mounted on a mounting region of the mirror.
9. The method of claim 1 , further comprising separating the transmitted component of the light beam from the scattered component of the light beam by a machined lens placed in a path of the light beam, a beam guidance or deflection unit mounted on a mounting region of the lens.
10. The method of claim 1 , wherein the step of measuring the intensity of the transmitted component of the light beam includes measuring the intensity with a detector having wavelength-selective components.
11. The method of claim 1 , wherein signals of both the scattered and transmitted components of the light beam are measured both separately and simultaneously.
12. The method of claim 1 , further including recording a signal of the transmitted component of the light beam as it passes through a vessel for accommodating a material to be measured as a function of a position of the vessel.
13. The method of claim 12 , wherein the vessel is a cuvette.
14. The method of claim 1 , further including setting, testing, and if appropriate, correction of the position of a vessel for accommodating a material to be measured, wherein the setting, testing, and correction includes moving the vessel through the light beam; scanning the vessel during its movement through the light beam; and recording a signal of the transmitted component of the light beam as a function of the vessel in order to define the position of the vessel relative to the light beam.
15. The method of claim 14 , wherein the vessel is a cuvette.
16. The method of claim 1 , wherein the method is used for in-process control for the purpose of validation in automatic diagnostic analyzers.
17. The method of claim 1 , wherein the method is used in analysis processes.
18. The method of claim 1 , wherein the method is used in in-vitro diagnosis processes.
19. The method of claim 1 , wherein the first detector is positioned between the accommodation vessel and the second detector and the diaphragm is positioned between the first detector and the accommodation vessel.
20. A method of calibrating a system for measuring a specimen using light, comprising:
directing a measuring light beam toward a detection unit;
passing an empty vessel for accommodating a material to be measured through the path of the measuring light beam via a rotor system;
separating a transmitted component of the measuring light beam from a scattered component of the measuring light beam;
measuring the intensity of a transmitted component of the light beam based on a position of the empty vessel relative to the light beam;
measuring the intensity of a scattered component of the light beam separately from the transmitted component based on the position of the empty vessel relative to the light beam;
adjusting an intensity of the light beam based on the measured intensity of the transmitted component of the light beam; and
allowing the scattered component of the light beam to pass around a diaphragm upon which the transmitted component of the light beam impinges.
21. The method of claim 20 , wherein the method is used in analysis processes.
22. The method of claim 20 , wherein the method is used in in-vitro diagnosis processes.
23. The method of claim 20 , wherein the first detector is positioned between the empty vessel and the second detector and the diaphragm is positioned between the first detector and the empty vessel.
24. A method of measuring a specimen using light, comprising:
calibrating a measuring system by:
directing a measuring light beam toward a detection unit;
passing an empty vessel for accommodating a material to be measured through the path of the measuring light beam via a rotor system;
separating a transmitted component of the measuring light beam from a scattered component of the measuring light beam;
measuring the intensity of a transmitted component of the light beam based on a position of the empty vessel relative to the light beam;
measuring the intensity of a scattered component of the light beam separately from the transmitted component based on the position of the empty vessel relative to the light beam;
adjusting an intensity of the measuring light based on the measured intensity of the transmitted component of the light beam; and
allowing the scattered component of the light beam to pass around a diaphragm upon which the transmitted component of the light beam impinges;
filling the empty vessel with the specimen to be measured;
placing the vessel containing the specimen to be measured in the path of the measuring light beam;
measuring the intensity of a transmitted component of the light beam; and
measuring the intensity of a scattered component of the light beam separately from the transmitted component.
25. The method of claim 24 , wherein the method is used in analysis processes.
26. The method of claim 24 , wherein the method is used in in-vitro diagnosis processes.
27. The method of claim 24 , wherein the first detector is positioned between the empty vessel and the second detector and the diaphragm is positioned between the first detector and the empty vessel.