Circovirus sequences associated with piglet weight loss disease (PWD)
View Patent ↗The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection is further provided. Pharmaceutical, including vaccines, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
1. A method for detecting and quantifying at least one antibody directed against a circovirus of type PCVB by an Enzyme-Linked Immunosorbent Assay (ELISA) method, wherein the at least one antibody is capable of binding to a polypeptide encoded by a nucleic acid having a nucleotide sequence with at least 90% sequence identity to SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27.
2. The method of claim 1 , wherein the method comprises adsorpting the circovirus of type PCVB onto a support material.
3. The method of claim 1 , wherein the method comprises depositing a polypeptide expressed by a porcine circovirus (PCV) in the wells of a microtiter plate.
4. The method of claim 1 , wherein the circovirus of type PCVB contains a nucleic acid having a nucleotide sequence with at least 90% sequence identity to SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27.
5. The method of claim 1 , wherein the circovirus of type PCVB contains a nucleic acid having the nucleotide sequence SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27.
6. The method of claim 1 , wherein the circovirus of type PCVB contains a nucleic acid having a nucleotide sequence SEQ ID NO: 23.
7. The method of claim 1 , wherein the circovirus of type PCVB contains a nucleic acid having a nucleotide sequence SEQ ID NO: 25.